Nanoparticle-Based Electrochemical Immunosensor for the Detection of Phosphorylated Acetylcholinesterase: An Exposure Biomarker of Organophosphate Pesticides and Nerve Agents

Details

• Personal Author:
  Barry R ; Gassman PL ; Lin Y ; Liu G ; Petersen C ; Timchalk C ; Wang J
• Description:
  A nanoparticle-based electrochemical immunosensor has been developed for the detection of phosphorylated acetylcholinesterase (AChE), which is a potential biomarker of exposure to organophosphate (OP) pesticides and chemical warfare nerve agents. Zirconia nanoparticles (ZrO(2) NPs) were used as selective sorbents to capture the phosphorylated AChE adduct, and quantum dots (ZnS@CdS, QDs) were used as tags to label monoclonal anti-AChE antibody to quantify the immunorecognition events. The sandwich-like immunoreactions were performed among the ZrO(2) NPs, which were pre-coated on a screen printed electrode (SPE) by electrodeposition, phosphorylated AChE and QD-anti-AChE. The captured QD tags were determined on the SPE by electrochemical stripping analysis of its metallic component (cadmium) after an acid-dissolution step. Paraoxon was used as the model OP insecticide to prepare the phosphorylated AChE adducts to demonstrate proof of principle for the sensor. The phosphorylated AChE adduct was characterized by Fourier transform infrared spectroscopy (FTIR) and mass spectroscopy. The binding affinity of anti-AChE to the phosphorylated AChE was validated with an enzyme-linked immunosorbent assay. The parameters (e.g., amount of ZrO(2) NP, QD-anti-AChE concentration,) that govern the electrochemical response of immunosensors were optimized. The voltammetric response of the immunosensor is highly linear over the range of 10 pM to 4 nM phosphorylated AChE, and the limit of detection is estimated to be 8.0 pM. The immunosensor also successfully detected phosphorylated AChE in human plasma. This new nanoparticle-based electrochemical immunosensor provides an opportunity to develop field-deployable, sensitive, and quantitative biosensors for monitoring exposure to a variety of OP pesticides and nerve agents. [Description provided by NIOSH]
• Subjects:
  Agriculture Chemical Warfare Agents Chemistry Hypersensitivity Immune System Insecticides Nervous System Occupational Health Organophosphates Safety
• Keywords:
  Agricultural-chemicals Biological-effects Chemical-analysis Chemical-hypersensitivity Chemical-properties Chemical-reactions Chemical-synthesis Chemical-warfare-agents Electrochemical-analysis Electrochemical-properties Electrochemical-reactions Immune-reaction Immunochemistry Nanotechnology Nerve-function Nervous-system-function Organo-phosphorus-compounds Organo-phosphorus-pesticides Parasympathetic-nervous-system Pesticides-and-agricultural-chemicals Qualitative-analysis

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• ISSN:
  0947-6539
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  32
• NIOSHTIC Number:
  nn:20035054
• Citation:
  Chemistry 2008 Mar; 14(32):9951-9959
• Contact Point Address:
  Yuehe Lin, Pacific Northwest National Laboratory, Richland, WA 99352
• Email:
  guodong.liu@ndsu.edu
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  True
• Start Date:
  20060901
• Source Full Name:
  Chemistry: A European Journal
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d86a580913d0da748e836be987c1fe53e5d0e518961f361f2474d87fc63ae7c55fb476cec9eeef3bc0843d4a8dc41641b1709b8992f3e9e5338a1f5099d46131
• Download URL:
  https://stacks.cdc.gov/view/cdc/187037/cdc_187037_DS1.pdf
• File Type:
  [PDF - 431.55 KB ]