The Fate of Inhaled Nanoparticles: Detection and Measurement by Enhanced Dark-Field Microscopy

Details

• Personal Author:
  Antonini JM ; Battelli LA ; Castranova, Vincent ; Hubbs, Ann F. ; Mercer RR ; Porter DW ; Qian Y ; Roberts, Jennifer R. ; Scabilloni JF ; Sisler JD ; Wang L
• Description:
  Assessing the potential health risks for newly developed nanoparticles poses a significant challenge. Nanometer-sized particles are not generally detectable with the light microscope. Electron microscopy typically requires high-level doses, above the physiologic range, for particle examination in tissues. Enhanced dark-field microscopy (EDM) is an adaption of the light microscope that images scattered light. Nanoparticles scatter light with high efficiency while normal tissues do not. EDM has the potential to identify the critical target sites for nanoparticle deposition and injury in the lungs and other organs. This study describes the methods for EDM imaging of nanoparticles and applications. Examples of EDM application include measurement of deposition and clearance patterns. Imaging of a wide variety of nanoparticles demonstrated frequent situations where nanoparticles detected by EDM were not visible by light microscopy. EDM examination of colloidal gold nanospheres (10-100 nm diameter) demonstrated a detection size limit of approximately 15 nm in tissue sections. EDM determined nanoparticle volume density was directly proportional to total lung burden of exposed animals. The results confirm that EDM can determine nanoparticle distribution, clearance, transport to lymph nodes, and accumulation in extrapulmonary organs. Thus, EDM substantially improves the qualitative and quantitative microscopic evaluation of inhaled nanoparticles. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Exposure Fibrosis Hazardous Substances Lung Occupational Health Pathology Respiratory System Risk Assessment Risk Factors Safety Toxicology

  More +
• Keywords:
  Author Keywords: Fibrosis Electron Microscopy Exposure Levels Gold Health Effects Health Hazards Inhalation Microscopy Nanoparticles Nanotechnology Risk Factors Tissue Culture

  More +
• ISSN:
  0192-6233
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  28-46
• Volume:
  46
• Issue:
  1
• NIOSHTIC Number:
  nn:20050464
• Citation:
  Toxicol Pathol 2018 Jan; 46(1):28-46
• Contact Point Address:
  Robert R. Mercer, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Drive, Morgantown, WV 26505
• Email:
  rmercer@cdc.gov
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Toxicologic Pathology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c20bce7bdc3378a6a9244dd2eb7b26099de88e4aaedda1cbad7815f1177446d124811239884c6d2f07f90f9ae61c92a0c199aead36e3dd374ebefdb7a6b76cec
• Download URL:
  https://stacks.cdc.gov/view/cdc/210791/cdc_210791_DS1.pdf
• File Type:
  [PDF - 3.28 MB ]