Social Isolation Impairs Oral Palatal Wound Healing in Sprague-Dawley Rats: A Role for miR-29 and miR-203 via VEGF Suppression

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Yang, Linglan ; Engeland, Christopher G. ; Cheng, Bin
• Description:
  Objective ; To investigate the effects of social isolation on oral mucosal healing in rats, and to determine if wound-associated genes and microRNAs (miRNAs) may contribute to this response. ; Methods ; Rats were group housed or socially isolated for 4 weeks before a 3.5 mm wound was placed on the hard oral palate. Wound closure was assessed daily and tissues were collected for determination of gene expression levels and miRNAs (i.e., miR-29a,b,c and miR-203). The predicted target of these microRNAs (i.e., vascular endothelial growth factor A, VEGFA) was functionally validated. ; Results ; Social isolation stress delayed the healing process of oral palatal mucosal wounds in rats. Lower mRNA levels of interleukin-1β (IL1β), macrophage inflammatory protein-1α (MIP1α), fibroblast growth factor 7 (FGF7), and VEGFA were found in the biopsied tissues of isolated animals on days 1 and/or 3 post-wounding. Intriguingly, the isolated rats persistently exhibited higher levels of miR-29 family members and miR-203. Our results confirmed that VEGFA is a direct target of these miRNAs, as both miR-29a,c and miR-203 strongly and specifically suppressed endogenous VEGFA expression in vitro. ; Conclusions ; This study in rats demonstrates for the first time that social isolation delays oral mucosal healing, and suggests a potential role for healing-associated gene and miRNA interactions during this process via modulation of VEGF expression.
• Subjects:
  • Animals
  • Base Sequence
  • Chemokine CCL3
  • Fibroblast Growth Factor 7
  • Gene Expression Regulation
  • Interleukin-1beta
  • Male
  • MicroRNAs
  • Molecular Sequence Data
  • Mouth Mucosa
  More +
• Source:
  PLoS One. 2013; 8(8).
• Document Type:
  Journal Article
• Volume:
  8
• Issue:
  8
• Download URL:
  https://stacks.cdc.gov/view/cdc/21060/cdc_21060_DS1.pdf
• File Type:
  [PDF - 835.63 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:1491b5f09104108979c7d88555360335dcaba0ee0469b9c7de1e4179577d44d5