Improving Reproducibility of Lab-on-a-Chip Sensor with Bismuth Working Electrode for Determining Zn in Serum by Anodic Stripping Voltammetry

Details

• Personal Author:
  Bange A ; Heineman WR ; Kang W ; Papautsky I ; Pei X ; Yue W
• Description:
  This work reports on the continuing development of a lab-on-a-chip electrochemical sensor for determination of zinc in blood serum using square wave anodic stripping voltammetry. The microscale sensor consists of a three electrode system, including an environmentally friendly bismuth working electrode, an integrated silver/silver chloride reference electrode, and a gold auxiliary electrode. The sensor demonstrates a linear response in 0.1 M acetate buffer at pH 6 for zinc concentrations in the 1-30 µM range. By optimizing bismuth film deposition and better control of the fabrication process, repeatability of the sensor was improved, reducing variability from 42% to <2%. Through optimization of electrolyte and stripping voltammetry parameters, limit of detection was greatly improved to 60 nM. The optimized sensor was also able to measure zinc in the extracted blood serum. Ultimately, with integrated sample preparation, the sensor will permit rapid (min) measurements of zinc from a sub-mL sample (a few drops of blood) for clinical applications. [Description provided by NIOSH]
• Subjects:
  Acetates Blood Occupational Health Safety Silver
• Keywords:
  Blood Serum Electrochemical Properties Electrochemistry Gold Silver Compounds Zinc
• ISSN:
  0013-4651
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  161
• Issue:
  2
• NIOSHTIC Number:
  nn:20050000
• Citation:
  J Electrochemi Soc 2014 Feb; 161(2):B3160-B3166
• Contact Point Address:
  Ian Papautsky, BioMicroSystems Laboratory, Department of Electrical Engineering and Computing Systems, University of Cincinnati, Cincinnati, Ohio 45221
• Email:
  ian.papautsky@uc.edu
• CAS Registry Number:
  Gold (CAS RN 7440-57-5) ; Silver (CAS RN 7440-22-4) ; Zinc (CAS RN 7440-66-6)
• Federal Fiscal Year:
  2014
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of the Electrochemical Society
• End Date:
  20260630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8ed6266d8447035326331b13e6786939cb0565ef2c75344a76ec2f8cd7fbf312b9663f2a828082a60c1002470fd2080aac8eabaf6b3dfdcf18be4c57c5d4b089
• Download URL:
  https://stacks.cdc.gov/view/cdc/210561/cdc_210561_DS1.pdf
• File Type:
  [PDF - 1.38 MB ]