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Fine Particulate Matter (PM2.5) Enhances Allergic Sensitization in BALB/C Mice



Details

  • Personal Author:
  • Description:
    Ambient particulate matter (PM), a component of air pollution, exacerbates airway inflammation and hyperreactivity in asthmatic patients. Studies showed that PM possesses adjuvant-like properties that enhance the allergic inflammatory response; however, the mechanism (or mechanisms) by which PM enhances the allergic response remains to be determined. The aim of this study was to assess how exposure to fine PM collected from Sacramento, California, shapes the allergic airway immune response in BALB/c mice undergoing sensitization and challenge with ovalbumin (OVA). Eight-week-old BALB/c male mice were sensitized/challenged with phosphate-buffered saline (PBS/PBS; n = 6), PM/PBS (n = 6), OVA/OVA (n = 6), or OVA + PM/OVA (n = 6). Lung tissue, bronchoalveolar lavage fluid (BALF), and plasma were analyzed for cellular inflammation, cytokines, immunoglobulin E, and heme oxygenase-1 (HO-1) expression. Mice in the OVA + PM/OVA group displayed significantly increased airway inflammation compared to OVA/OVA animals. Total cells, macrophages, and eosinophils recovered in BALF were significantly elevated in the OVA + PM/OVA compared to OVA/OVA group. Histopathological grading indicated that OVA + PM/OVA treatment induced significant inflammation compared to OVA/OVA. Both immunoglobulin (Ig) E and tumor necrosis factor (TNF) alpha levels were significantly increased in OVA/OVA and OVA + PM /OVA groups compared to PBS/PBS control. The number of HO-1 positive alveolar macrophages was significantly elevated in lungs of mice treated with OVA + PM /OVA compared to OVA/OVA. Our findings suggest that fine PM enhances allergic inflammatory response in pulmonary tissue through mechanisms involving increased oxidative stress. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    1528-7394
  • Document Type:
    Text
  • Funding:
    Cooperative Agreement
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Pages in Document:
    197-207
  • Volume:
    80
  • Issue:
    4
  • NIOSHTIC Number:
    nn:20049754
  • Citation:
    J Toxicol Environ Health A 2017 May; 80(4):197-207
  • Contact Point Address:
    Kent E. Pinkerton, Center for Health and the Environment, University of California, 1 Shields Ave., Davis, CA 95616, USA
  • Email:
    kepinkerton@ucdavis.edu
  • Federal Fiscal Year:
    2017
  • NORA Priority Area:
    Agriculture, Forestry and Fishing
  • Performing Organization:
    University of California - Davis
  • Peer Reviewed:
    True
  • Start Date:
    20010930
  • Source Full Name:
    Journal of Toxicology and Environmental Health, Part A: Current Issues
  • End Date:
    20270929
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:30b33987666fbdb2cfd1c383abb43289773b0fdaa226f21a879f54c78f9dcfcd0191a29787efc8dd4447ff7aeb81ddebb0ea7cc1aee43ebb1c9d8ffd057c297e
  • Download URL:
  • File Type:
    Filetype[PDF - 1.24 MB ]
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