Evaluation of Pulmonary Effects of 3-D Printer Emissions from Acrylonitrile Butadiene Styrene Using an Air-Liquid Interface Model of Primary Normal Human-Derived Bronchial Epithelial Cells (Dataset)

Details

• Personal Author:
  Battelli L ; Bowers L ; Coyle J ; Farcas MT ; Friend S ; Hammer MA ; Hammond, Duane R. ; Kashon ML ; Mandler W ; Matheson J ; McKinney W ; Orandle MS ; Qi C ; Qian Y ; Richardson D ; Service S ; Stefaniak, Aleksandr B. ; Thomas TA
• Description:
  This study investigated the inhalation toxicity of the emissions from 3-D printing with acrylonitrile butadiene styrene (ABS) filament using an air-liquid interface (ALI) in vitro model. Primary normal human-derived bronchial epithelial cells (NHBEs) were exposed to ABS filament emissions in an ALI for 4 h. The mean and mode diameters of ABS emitted particles in the medium were 175 +/- 24 nm and 153 +/- 15 nm, respectively. The average particle deposition per surface area of the epithelium was 2.29 × 107 +/- 1.47 × 107 particle/cm2, equivalent to an estimated average particle mass of 0.144 +/- 0.042 µg/cm2. [Description provided by NIOSH]
• Subjects:
  Butadienes Cells, Cultured Cytotoxins Hazardous Substances Occupational Health Particulate Matter Printing, Three-Dimensional Respiratory System Safety Styrene
• Keywords:
  3D Printing Acrylonitrile Cell Cultures Cytotoxicity Emission Sources Inhalation In Vitro Study Particulates Styrenes Three Dimensional Printing Toxins

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• DOI:
  https://doi.org/10.26616/NIOSHRD-1044-2022-0
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Dataset
• Genre:
  Research Dataset
• Place as Subject:
  Maryland ; Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division ; DFSE - Division of Field Studies and Engineering
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20066033
• Citation:
  Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1044-2022-0, 2022 Sep; :dataset
• Contact Point Address:
  Pathology and Physiology Research Branch (PPRB), Health Effects Laboratory Division (HELD), National Institute for Occupational Safety and Health (NIOSH), 1095 Willowdale Road, Morgantown, WV 26505. Phone: 304-285-5831
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Manufacturing ; Construction
• Peer Reviewed:
  False
• Source Full Name:
  Evaluation of pulmonary effects of 3-D printer emissions from acrylonitrile butadiene styrene using an air-liquid interface model of primary normal human-derived bronchial epithelial cells
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2a5ae0b792cc699af7fffd53c62e39a38983133eb1d7f6c1b369dd940f31889d56533ec2baeb14fba5046863425cec0b251dca599ea0857fca79b4a4be3c1658
• Download URL:
  https://stacks.cdc.gov/view/cdc/209117/cdc_209117_DS1.pdf
• File Type:
  [PDF - 494.71 KB ]