Impact of Vitamin D on Hyperoxic Acute Lung Injury in Neonatal Mice

Details

• Personal Author:
  Alcorn JL ; Crocker CE ; Davies J ; Johnston RA ; Karmouty-Quintana H ; Khan AM ; Li H ; Tran TT
• Description:
  Background: Prolonged exposure to hyperoxia can lead to hyperoxic acute lung injury (HALI) in preterm neonates. Vitamin D (VitD) stimulates lung maturation and acts as an anti-inflammatory agent. Our objective was to determine if VitD provides a dose-dependent protective effect against HALI by reducing inflammatory cytokine expression and improving alveolarization and lung function in neonatal mice. Methods: C57BL/6 mouse neonates were randomized and placed in room air or hyperoxic (85% O2) conditions for 6 days. Control, low (5 ng/neonate) and high (25 ng/neonate) doses of VitD were administered daily beginning at day 2 via oral gavage. Lung tissue was analyzed for edema, changes in pulmonary structure and function, and inflammatory cytokine expression. Results: Neonatal mice treated with VitD in hyperoxic conditions had improved weight gain, reduced pulmonary edema and increased alveolar surface area compared to untreated pups in hyperoxia. No significant changes in cytokine expression were observed between untreated and VitD neonates. While changes in surfactant protein mRNA expression were impacted by hyperoxia and VitD administration, no significant changes in alveolar type II cell percentages were observed. At 3 weeks, mice in hyperoxia treated with VitD had greater lung compliance, diminished airway reactivity and improved weight gain. Conclusions: High dose VitD significantly limited harmful effects of HALI. These results suggest that supplementation of VitD to neonatal mice during hyperoxia exposure provides both short-term and long-term protective benefits against HALI. [Description provided by NIOSH]
• Subjects:
  Immune System Infant, Newborn Lung Occupational Health Respiratory System Safety
• Keywords:
  Acute Lung Injury Airway Responsiveness Author Keywords: Hyperoxia Cytokines HALI Immune Reaction Infammatory Cytokines Lung Function Neonates Oxygen Levels Preterm Neonate Vitamin D Vitamins

  More +
• ISSN:
  1471-2466
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Dallas Region [OSHA] ; Philadelphia Region [OSHA] ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  24
• NIOSHTIC Number:
  nn:20070337
• Citation:
  BMC Pulm Med 2024 Nov; 24:584
• Contact Point Address:
  Thu T. Tran, Division of Neonatology, Department of Pediatrics, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX, 77030, USA
• Email:
  Thu.Tran@uth.tmc.edu
• Federal Fiscal Year:
  2025
• Peer Reviewed:
  True
• Source Full Name:
  BMC Pulmonary Medicine
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:75e0a5de7284343048fe937ca2748dec557fccbb4060823b8831e401ff0cefe2c3a13b930f819b3b27a47fb48416cacbf6d462eb49a9fa38a1b2e586907fa771
• Download URL:
  https://stacks.cdc.gov/view/cdc/208570/cdc_208570_DS1.pdf
• File Type:
  [PDF - 2.90 MB ]