Chemerin Deficiency Exacerbates Ozone-Induced Increases in Airway Responsiveness

Details

• Personal Author:
  Boots T ; Johnston R ; Pilkington A ; Takahashi M ; Takahashi Y
• Description:
  Rationale: Intranasal insufflation of chemerin, a non-chemokine chemoattractant, decreases the severity of airway hyperresponsiveness (AHR) following antigen sensitization and challenge, an atopic asthma stimulus, via suppression of CCL2 secretion. However, the effect of chemerin on AHR induced by ozone (O3), a non-atopic asthma stimulus, is unknown. Furthermore, chemerin reduces alignment of collagen, and when collagen is present in excess in the lung interstitium, this decreases quasi-static respiratory system compliance (Cstat). Therefore, based on these observations, we hypothesized that Cstat is decreased and the severity of O3-induced AHR increased in mice genetically deficient in chemerin (chemerin-deficient mice). Methods: Wild-type (C57BL/6J) and chemerin-deficient mice were exposed to filtered room air (air) or O3 (2 parts/million) for three hours. Twenty-four hours following cessation of exposure, Cstat was calculated from the deflationary limb of pressure-volume curves obtained from air-exposed mice while responses to methacholine for respiratory system resistance (RRS) and compliance (CRS) were determined in air- or O3-exposed mice using the forced oscillation technique. Results: Cstat was reduced in chemerin-deficient as compared to wild-type mice following air exposure. Compared to genotype-matched, air-exposed controls, responses to methacholine for RRS and CRS were significantly increased and decreased, respectively, following O3 exposure. However, increases in RRS and decreases in CRS following O3 exposure were exacerbated in chemerin-deficient as compared to wild-type mice. Conclusions: In chemerin-deficient mice, decreases in Cstat may result from genotype-related differences in lung collagen alignment while increases in the severity of O3-induced AHR may be a consequence of the inability to suppress CCL2 secretion in the lung. [Description provided by NIOSH]
• Subjects:
  Asthma Asthma, Occupational Immune System Occupational Health Ozone Respiratory System Safety
• Keywords:
  Airway Resistance Immune Reaction Inhalation Studies Physiopathology
• ISSN:
  0091-6749
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  151
• Issue:
  2
• NIOSHTIC Number:
  nn:20070241
• Citation:
  J Allergy Clin Immunol 2023 Feb; 151(2)(Suppl):AB68
• CAS Registry Number:
  Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  2023
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:15a99bfeec51ecc2bf3bd61e992c79e81ecdebd3317cc5b4b9e263ed114cd0264c9122ef120f6c0d3d71d99d23345c6c11c5a552f57080ee5bd6e5b51cf64028
• Download URL:
  https://stacks.cdc.gov/view/cdc/208490/cdc_208490_DS1.pdf
• File Type:
  [PDF - 103.76 KB ]