Transcriptional and Morphological Responses Following Distinct Muscle Contraction Protocols for Snell Dwarf (Pit1dw/Dw) Mice

Details

• Personal Author:
  Baker BA ; McKinstry KA ; Rader EP
• Description:
  The Snell dwarf mouse (Pit1dw/dw), an animal model of congenital combined pituitary hormone deficiency, displays skeletal muscle weakness. While enhanced responsivity to repeated exposures of muscle contractions have been documented for Snell dwarf mice, the response following single exposure to distinct contraction protocols remained uncharacterized. The purpose of this study was to investigate the muscle recovery of Snell dwarf and control littermate mice following a single exposure to two separate protocols-an intermittent slow velocity (30 degrees/s) contraction protocol or a continuous rapid velocity (500 degrees/s) contraction protocol. Following both protocols for control mice, torque values were 30% and 80% of pre-protocol values at 5 min and 3 days, respectively. At 10 days, performance returned to baseline for the 30 degrees/s protocol and were depressed for the 500 degrees/s protocol. For Snell dwarf mice following both protocols, torques were depressed to 5% of pre-protocol values at 5 min and returned to baseline by 3 days. Recovery following the 30 degrees/s protocol for control mice and both protocols for Snell dwarf mice coincided with increased transcriptional output, upregulation of cytokine-mediated signaling genes, and a distribution shift to smaller muscle fibers with reduced area per nucleus. These features represent efficacious remodeling ubiquitous across distinct contraction paradigms in the context of the Pit1 mutation. [Description provided by NIOSH]
• Subjects:
  Accident Prevention Accidents Accidents, Occupational Coal Coal Mining Mining Occupational Health Safety
• Keywords:
  Animal Studies Author Keywords: Plantarflexor Muscles Growth Disorders Muscle Function Muscles Pituitary Gland Disorders Skeletal Muscle Skeletal System Stretch-shortening Contractions
• ISSN:
  2051-817X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  12
• Issue:
  17
• NIOSHTIC Number:
  nn:20070124
• Citation:
  Physiol Rep 2024 Sep; 12(17):e70027
• Contact Point Address:
  Erik P. Rader, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, MS L3014, 1095 Willowdale Rd., Morgantown 26505, WV, USA
• Email:
  wlz4@cdc.gov
• Federal Fiscal Year:
  2024
• Peer Reviewed:
  True
• Source Full Name:
  Physiological Reports
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:04310625567140583f19768bede17e809ac2be1610cf748be23ac9f7d147cae91fd393b950737fdf5a2b3fd771449da3f3541fa909b21557761eeacda6878ca6
• Download URL:
  https://stacks.cdc.gov/view/cdc/208409/cdc_208409_DS1.pdf
• File Type:
  [PDF - 6.99 MB ]