Single-Cell RNA Sequencing Data Identify a Conserved Population of Metallothionein-Expressing Macrophages That May Be Ubiquitous in Vital Human Organs

Details

• Personal Author:
  Aloman C ; Azzara AV ; Branch AD ; Cao L ; Daccache JA ; Eng F ; Guarnieri P ; Herron D ; Ma N ; Miller M ; Pullen SS ; Schiano T ; Ward SC
• Description:
  We used publicly available single-cell RNA sequencing data to identify conserved tissue macrophage populations in human organs. Among the subsets, we found a rare population of metallothionein-expressing macrophages that are present in all vital organs analyzed. We deeply phenotype this subset and determine their localization in the human liver. ... In conclusion, using publicly available scRNA-sequencing data and immunohistochemistry, we transcriptionally profiled MAC_MTs, delineated shared marker genes of MAC_MTs, established their presence in transcriptomic data from multiple vital organs, identified predictors of MAC_MT function, and determined their localization in human liver. Our research suggests that MAC_MTs occur in the majority of vital human organs. Trajectory analysis suggests they differentiate locally within their resident organ, consistent with evidence that corneal tissue upregulates MT genes when cultured with monocyte-derived macrophages. Our work sets the stage for mechanistic studies defining the functional role of MAC_MTs in tissue homeostasis and remodelling. [Description provided by NIOSH]
• Subjects:
  Digestive System Digestive System Diseases Genetics Immune System Liver Occupational Health Safety
• Keywords:
  Genes Immune Reaction Immunochemistry Immunology Liver Disorders Liver Tissue
• ISSN:
  2768-0622
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Boston Region [OSHA] ; Connecticut ; New York ; New York City Region [OSHA]
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  4
• Issue:
  5
• NIOSHTIC Number:
  nn:20070103
• Citation:
  Clin Transl Discov 2024 Oct; 4(5):e348
• Contact Point Address:
  Andrea D. Branch, Department of Medicine, Division of Liver Disease, Icahn School of Medicine at Mount Sinai, New York, NY, USA
• Email:
  Andrea.Branch@mssm.edu
• Federal Fiscal Year:
  2025
• Performing Organization:
  Icahn School of Medicine at Mount Sinai, New York
• Peer Reviewed:
  False
• Start Date:
  20170701
• Source Full Name:
  Clinical and Translational Discovery
• End Date:
  20240630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:35d82a85b0f17328255313d2cf5c606af9080e8098d9423f1c40194be811584eb4f6f5063bc3cf0aaf8d6d73994e698f65b3d229ea882ecd430f31c5b9a1e41e
• Download URL:
  https://stacks.cdc.gov/view/cdc/208396/cdc_208396_DS1.pdf
• File Type:
  [PDF - 6.31 MB ]