Maternal Nano-Titanium Dioxide Inhalation Exposure Alters Placental Cyclooxygenase and Oxidant Balance in a Sexually Dimorphic Manner

Details

• Personal Author:
  Bowdridge EC ; Dunn AC ; Goldsmith WT ; Griffith JA ; Kelley EE ; King RD ; Lewis SE ; Maxwell BA ; Nurkiewicz TR
• Description:
  The placenta plays a critical role in nutrient-waste exchange between the maternal and fetal circulation, and thus impacts fetal growth and development. We have previously shown that nano-titanium dioxide (nano-TiO2) inhalation exposure during gestation decreased fetal female pup and placenta mass [1], which persists in the following generation [2]. In utero exposed females, once mated, their offspring's placentas had increased capacity for H2O2 production. Generation of oxidants such as hydrogen peroxide (H2O2), have been shown to impact cyclooxygenase activity, specifically metabolites such as prostacyclin (PGI2) or thromboxane (TXA2). Therefore, we hypothesized that maternal nano-TiO2 inhalation exposure during gestation results in alterations in placental production of prostacyclin and thromboxane mediated by enhanced H2O2 production in a sexually dimorphic manner. Pregnant Sprague-Dawley rats were exposed to nano-TiO2 aerosols or filtered air (sham-control) from gestational day (GD) 10-19. Dams were euthanized on GD 20, and fetal serum and placental tissue were collected based on fetal sex. Fetal placental zones (junctional zone (JZ) and labyrinth zone (LZ)) were assessed for xanthine oxidoreductase (XOR) activity, H2O2, and catalase activity, as well as 6-keto-PGF1a and TXB2 levels. Nano-TiO2 exposed fetal female LZ demonstrated significantly greater XOR activity compared to exposed males. Exposed fetal female LZ also demonstrated significantly diminished catalase activity compared to sham-control females. Exposed fetal female LZ had significantly increased abundance of 6-keto-PGF1a compared to sham-control females and increased TXB2 compared to exposed males. In the aggregate these data indicate that maternal nano-TiO2 inhalation exposure has a greater impact on redox homeostasis and PGI2/TXA2 balance in the fetal female LZ. Future studies need to address if treatment with an XO inhibitor during gestation can prevent diminished fetal female growth during maternal nano-TiO2 inhalation exposure. [Description provided by NIOSH]
• Subjects:
  Aerosols Occupational Health Pregnancy Respiratory System Safety Sex Characteristics Titanium Toxicology
• Keywords:
  Author Keywords: Titanium Dioxide Inhalation Nanoparticles Nanotoxicology Oxidants Prenatal Exposure Prostacyclin Sex Factors Sexual Dimorphism Thromboxane Titanium Dioxide Xanthine Oxidoreductase

  More +
• ISSN:
  2667-1379
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  10
• NIOSHTIC Number:
  nn:20069602
• Citation:
  Adv Redox Res 2024 Apr; 10:100090
• Contact Point Address:
  Elizabeth C. Bodridge, Department of Physiology, Pharmacology, and Toxicology, University of West Virginia, School of Medicine, 3076 Health Sciences Center North, PO Box 9229, Morgantown, WV 26506-9229, USA
• Email:
  ebowdrid@hsc.wvu.edu
• CAS Registry Number:
  Titania (CAS RN 13463-67-7)
• Federal Fiscal Year:
  2024
• Performing Organization:
  West Virginia University
• Peer Reviewed:
  True
• Start Date:
  20220901
• Source Full Name:
  Advances in Redox Research
• End Date:
  20230831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:728ebb77b6816920a63f3b675718bbea9ad252b22f40082b1dbc30ec2e3f9aa774b5a020504ecab9555ab0487766c3e221fbd3a45bc251147ae6065cbda6ddf6
• Download URL:
  https://stacks.cdc.gov/view/cdc/208002/cdc_208002_DS1.pdf
• File Type:
  [PDF - 1.96 MB ]