Palm-Sized Lab-in-a-Magnetofluidic Tube Platform for Rapid and Sensitive Virus Detection

Details

• Personal Author:
  Avery L ; Banach D ; Li Z ; Liu C ; Zhang J ; Zhang S ; Zhao H
• Description:
  Simple, sensitive, and accurate molecular diagnostics are critical for preventing rapid spread of infection and initiating early treatment of diseases. However, current molecular detection methods typically rely on extensive nucleic acid sample preparation and expensive instrumentation. Here, a simple, fully integrated, lab-in-a-magnetofluidic tube (LIAMT) platform is presented for "sample-to-result" molecular detection of virus. By leveraging magnetofluidic transport of micro/nano magnetic beads, the LIAMT device integrates viral lysis, nucleic acid extraction, isothermal amplification, and CRISPR detection within a single engineered microcentrifuge tube. To enable point-of-care molecular diagnostics, a palm-sized processor is developed for magnetofluidic separation, nucleic acid amplification, and visual fluorescence detection. The LIAMT platform is applied to detect SARS-CoV-2 and HIV viruses, achieving a detection sensitivity of 73.4 and 63.9 copies µL-1, respectively. Its clinical utility is further demonstrated by detecting SARS-CoV-2 and HIV in clinical samples. This simple, affordable, and portable LIAMT platform holds promise for rapid and sensitive molecular diagnostics of infectious diseases at the point-of-care. [Description provided by NIOSH]
• Subjects:
  Diagnosis Occupational Health Safety Viral Diseases
• Keywords:
  Author Keywords: CRISPR-based Nucleic Acid Detection Diagnostic Tests Emerging Technology HIV Detection Magnetofluidic Separation Microfluidic Technology Molecular Biology Nucleic Acids SARS-CoV-2 Detection Viral Infections
• ISSN:
  2198-3844
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; Nevada ; OSHA Region 1 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  11
• Issue:
  23
• NIOSHTIC Number:
  nn:20069579
• Citation:
  Adv Sci 2024 Jun; 11(23):2310066
• Contact Point Address:
  Changchun Liu, Department of Biomedical Engineering University of Connecticut Health Center Farmington, Connecticut 06030
• Email:
  chaliu@uchc.edu
• Federal Fiscal Year:
  2024
• Performing Organization:
  University of Nevada, Las Vegas
• Peer Reviewed:
  True
• Start Date:
  20210930
• Source Full Name:
  Advanced Science
• End Date:
  20230929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b5e451b47f951fc5fd36bb545e50f1955e4945ec278cc078cec5a8a429da02dc0de624ab44cf5394a05ed155f4843b27f1211a2af46e1538147afaea517ff760
• Download URL:
  https://stacks.cdc.gov/view/cdc/207986/cdc_207986_DS1.pdf
• File Type:
  [PDF - 3.99 MB ]