The Impact of LCN2 in the Lung Inflammatory Response to Agricultural Dust Exposure

Details

• Personal Author:
  Guardado ES
• Description:
  Agricultural workers have increased risks of developing debilitating respiratory conditions such as chronic obstructive pulmonary disease (COPD) due to their repetitive exposure to organic dust particles. Lipocalin-2 (LCN-2) is an acute phase protein that plays crucial roles in mediating immune responses to inflammatory stimuli in multiple tissues. Its role in the lung immune response to inflammatory stimuli other than bacteria, however, has not been well studied. This research examines the paradoxical roles of LCN2 in the immune response to extracts of dusts collected from swine confinement facilities (DE), in an effort to identify protective vs. harmful roles of LCN2 in the context of lung inflammation. Additionally, the cross-talk between LCN-2 and the anti-inflammatory cytokine IL-10 in mediating the tissue repair process was also examined. Major research findings indicate an important function of LCN-2 in regulating tissue homeostasis, tissue repair, and anti-inflammatory cytokine interleukin-10 (IL-10) production in the lungs. Additionally, compartmentalized effects on T cell and macrophage levels were observed in BALF vs. lung tissue in DE-exposed LCN-2 KO mice versus wildtype mice. The role of the LCN-2/IL-10 axis in lung tissue repair was further explored by administering recombinant IL-10 to LCN-2 KO mice, resulting in reduced pathology in the lungs. Ablation of LCN-2 resulted in deficits in recovery of mice exposed to DE, which was reversed with administration of IL-10. Lastly, at the population level, we show that higher bronchitis diagnosis rates are associated with natural dust exposure, and that intake of Omega-3 fatty acids is associated with decreased reports of bronchitis. Taken together, these investigations highlight a novel role for LCN-2 in promoting lung repair following organic dust-induced inflammation, mediated in part by IL-10. These findings may substantially impact the breadth of knowledge available for researchers in this field, and consequently help discriminate between the protective and detrimental roles exerted by LCN2 in response to inflammation in the lungs. This can further lead to the potential use of LCN-2 as a diagnostic marker or its therapeutic use in the treatment of debilitating lung inflammatory conditions. [Description provided by NIOSH]
• Subjects:
  Agriculture Dust Immune System Lung Occupational Health Respiratory System Safety
• Keywords:
  Agricultural Workers Cytokines Dust Exposure Immune Reaction Lung Irritants Organic Dusts
• Publisher:
  University of California, Riverside
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Dissertation
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-112
• NIOSHTIC Number:
  nn:20068747
• Citation:
  Riverside, CA: University of California, Riverside, 2022 Sep; :1-112
• Federal Fiscal Year:
  2022
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  False
• Start Date:
  20010930
• Source Full Name:
  The impact of LCN2 in the lung inflammatory response to agricultural dust exposure
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:82117a6178b0c3577e039352795c0fd7f59d73563274f667effd3d71cc9aa361875fb259fa997a90753f618ab0770cb9aeb345ae0784fae30c4a9992b4117ab9
• Download URL:
  https://stacks.cdc.gov/view/cdc/207331/cdc_207331_DS1.pdf
• File Type:
  [PDF - 8.47 MB ]