Triphenyl phosphite-induced ultrastructural changes in bovine adrenomedullary chromaffin cells

Details

• Personal Author:
  Abou-Donia MB ; Jones K ; Knoth-Anderson J ; Lapadula DM ; Veronesi B
• Description:
  Transmission electron microscopy (TEM) and scanning electron microscopy (TEM) were used to assess the morphological changes in primary cultures of bovine adrenomedullary chromaffin cells treated with triphenyl-phosphite (101020) (TPP). The cells were isolated and cultured with 100 micromolar (microM) TPP, O,O- diisopropylphosphorofluoridate (55914) (DFP), paraoxon (311455), or vehicle for 24 or 1 hour prior to fixing and TEM examination. For SEM, the cells were similarly treated for 24 hours. Radiolabeled adenosine incorporation into ATP was assessed through 0.25, 0.5, 1, 4, or 24 hour treatments with 100microM of the chemicals and thin layer chromatography. After 24 hours, almost all mitochondria were disrupted or swollen; glycogen buildup was evident. SEM revealed low viability for TPP treated cells. Filopodia were contracted, surface texture was irregular, and giant globular bodies were present. Paraoxon and DFP did not cause the same ultrastructural effects. The authors conclude that TPP's effect is specific and that its target is the mitochondria. [Description provided by NIOSH]
• Subjects:
  Biochemistry Cells, Cultured Cytotoxins Microbiology Nervous System Occupational Health Organophosphates Safety
• Keywords:
  Biochemical-tests Cell-cultures Cell-morphology Cytotoxic-effects Microscopic-analysis Neurotoxic-effects NIOSH-Grant NIOSH-Publication Nucleosides Organo-phosphorus-compounds
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  110-119
• Volume:
  112
• Issue:
  1
• NIOSHTIC Number:
  nn:00205254
• Citation:
  Toxicol Appl Pharmacol 1992 Jan; 112(1):110-119
• Contact Point Address:
  Pharmacology Duke University Department of Pharmacology Durham, N C 27710
• CAS Registry Number:
  Diisopropyl fluorophosphate (CAS RN 55-91-4) ; Paraoxon (CAS RN 311-45-5) ; Triphenyl phosphite (CAS RN 101-02-0)
• Federal Fiscal Year:
  1992
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e731d5d92036d0258442fc99da38581ff66487512b68ea5077e0ec47048a998fe1e498dc688fc4909893e84101647eec738687a7cf80c89ec9330a9dc153e3b4
• Download URL:
  https://stacks.cdc.gov/view/cdc/205813/cdc_205813_DS1.pdf
• File Type:
  [PDF - 13.02 MB ]