Tissue distribution of monochloroacetic acid and its binding to albumin in rats

Details

• Personal Author:
  Ansari GA ; Bhat HK ; Kaphalia BS ; Khan MF
• Description:
  The distribution patterns of monochloroacetic-acid (79118) (CA) were examined in rats. Male Sprague-Dawley-rats were administered a single oral dose of carbon-14 labeled CA at 0.1 millimole/kilogram by gavage. Rats were then placed in metabolic cages, and urine was collected at 4, 8, 12, 24, and 48 hours after dosing. Selected rats were sacrificed at each time point, and organs were analyzed for CA distribution. Rats in another group were treated with 1 millimole/kilogram CA daily by gavage for 3 days, and sacrificed 24 hours after the last dose. Results indicated that CA was rapidly absorbed and excreted from the body. The elimination phase appeared to be fast for intestine and kidney as compared to other tissues. Maximum radioactivity was detected in intestine and kidney at 4 and 8 hours after treatment; in decreasing order, radioactivity was also detected in liver, spleen, testes, lung, brain and heart. Examination of carbon-14 labeling in dialyzed plasma suggested that CA bound to plasma proteins in-vivo; albumin accounted for about 65% of the accumulation as determined by affinity chromatography. The authors suggest that isolation and identification of the adducts of amino acids will assist in understanding the mechanism of CA toxicity and the development of a suitable biomarker of CA exposure. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Hydrocarbons Laboratories Occupational Health Safety Urine
• Keywords:
  Chlorinated-hydrocarbons Grants-other Laboratory-animals Metabolic-study NIOSH-Grant NIOSH-Publication Protein-chemistry Tissue-distribution Urinalysis
• ISSN:
  0748-2337
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  53-61
• Volume:
  8
• Issue:
  1
• NIOSHTIC Number:
  nn:00206033
• Citation:
  Toxicol Ind Health 1992 Jan; 8(1-2):53-61
• Contact Point Address:
  Human Biol Chem and Genetics University of Texas Med BR Dept of Human Biol Chem&gene Galveston, Tex 77550-2774
• CAS Registry Number:
  Chloroacetic acid (CAS RN 79-11-8)
• Federal Fiscal Year:
  1992
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  University of Texas Medical Branch, Galveston, Texas
• Peer Reviewed:
  True
• Start Date:
  19860101
• Source Full Name:
  Toxicology and Industrial Health
• End Date:
  19960630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d217085ebe202e897eaadb495d364441e397b18aa61071edcc74297a1f15ed1800e5d03798766a9ba44fffc0a6da17fb374c3a33b7f0547f9edd61ceacc72de2
• Download URL:
  https://stacks.cdc.gov/view/cdc/205799/cdc_205799_DS1.pdf
• File Type:
  [PDF - 465.83 KB ]