The in vitro effects of alkanes, alcohols, and ketones on rat lung cytochrome P450-dependent alkoxyphenoxazone dealkylase activities

Details

• Personal Author:
  Judy DJ ; Rabovsky J
• Description:
  The effects of 21 aliphatic hydrocarbons on enzyme activities in lung microsomes from male Sprague-Dawley-rats were investigated. The specific enzymes considered were benzyloxyphenoxazone-dealkylase (BzOPhase) and ethoxyphenoxazone-dealkylase (EtOPhase). The rats were injected once with beta-naphthoflavone (BNF) at 80mg/kg 48 hours prior to sacrifice. The BzOPhase mediated release of resorufin was inhibited by the straight chain alkanes with 6 to 11 carbons. This inhibition was directed at the cytochrome-P450 dependent monooxygenase activity. Up to concentrations of 10 millimolar (mM) the straight chain alkanes did not affect EtOPhase activity. In branched heptane and hexane compounds the position of the methyl group affected the inhibiting potential with 3- methylheptane (589811), 4-methylheptane (589537) and 2,5- dimethylhexane (592132) causing 80 percent inhibitions. EtOPhase activity was not inhibited by the branched chain aliphatics. A decreased sensitivity of BzOPhase was demonstrated toward alcohol and carbonyl substituted hexanes. Some inhibitory potential was displayed toward the lung microsomal EtOPhase activity by 2-hexanol (626937) and 2-hexanone (591786). Octanols and octanones increased the 50 percent inhibition values toward BzOPhase compared to the unsubstituted normal octane. Octanol and octanone compounds inhibited EtOPhase activity as well. [Description provided by NIOSH]
• Subjects:
  Alkanes Butanones Environmental Exposure Environmental Pollution Enzymes Hydrocarbons Laboratories Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety

  More +
• Keywords:
  Alcohols Author Keywords: Cytochrome P450 Environmental-contamination Environmental-pollution Enzyme-activity Ketones Laboratory-animals Liver-microsomes Lung-cells Microsomal-enzymes NIOSH-Author Pulmonary-system-disorders

  More +
• ISSN:
  0340-5761
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  13-17
• Volume:
  63
• Issue:
  1
• NIOSHTIC Number:
  nn:00187696
• Citation:
  Arch Toxicol 1989; 63(1):13-17
• CAS Registry Number:
  (±)-2-Hexanol (CAS RN 626-93-7) ; 2,5-Dimethylhexane (CAS RN 592-13-2) ; 2-Hexanone (CAS RN 591-78-6) ; 3-Methylheptane (CAS RN 589-81-1) ; 4-Methylheptane (CAS RN 589-53-7)
• Federal Fiscal Year:
  1989
• NORA Priority Area:
  Pulmonary System Disorders
• Peer Reviewed:
  True
• Source Full Name:
  Archives of Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:49eaccb2a22b4edee88f28b9021e534e1d0cca942cc12763868d514ca68b58aa7db6dd884da1b6248076994effbf9a3c8ce62672394638b01e675b7fcd961476
• Download URL:
  https://stacks.cdc.gov/view/cdc/205733/cdc_205733_DS1.pdf
• File Type:
  [PDF - 525.82 KB ]