Studies on three structurally related phenylenediamines with the mouse micronucleus assay system

Details

• Personal Author:
  Nath J ; Ong T ; Shi X ; Soler-Niedziela L
• Description:
  An investigation was conducted to determine if the micronucleus assay can distinguish between the carcinogen 4-chloro-o- phenylenediamine (5131602) (COP) and the two noncarcinogens 4-nitro- phenylenediamine (5307142) (NOP) and p-phenylenediamine- dihydrochloride (615281) (PPD). Male CD-1-mice were treated intraperitoneally with 400mg/kg COP, 500mg/kg NOP, and 100mg/kg PPD. Sampling was performed 24, 48, or 72 hours after injection. COP induced significant dose related increases in micronucleus frequencies at all three sampling intervals, with the peak response occurring at 24 hours. At that time the highest dose tested, 400mg/kg, induced 7.4 micronucleated polychromatic erythrocytes (MPCE) per 1000 polychromatic erythrocytes (PCE) compared to 1.9 MPCE of the control. Values for PCE/normochromatic erythrocyte (NCE) ratios in mice treated with COP were not significantly different from controls. Mice treated with NOP did not exhibit any dose related response in micronuclei induction over the three dose levels tested when sampled at 24, 48, or 72 hours. PPD did not cause a significant dose or sampling time related response in micronuclei induction. Ratios of PCE/NCE in bone marrow cells of mice exposed to PPD exhibited significant decreases at the highest dose, 100mg/kg, at sampling times of 24 and 72 hours. [Description provided by NIOSH]
• Subjects:
  Chromosomes Cytotoxins Laboratories Mutagens Occupational Health Safety
• Keywords:
  Cell-damage Chromosome-damage Chromosome-disorders Cytotoxic-effects Diamines Genotoxic-effects In-vivo-study Laboratory-animals Mutagenesis NIOSH-Author
• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  43-48
• Volume:
  259
• Issue:
  1
• NIOSHTIC Number:
  nn:00200333
• Citation:
  Mutat Res 1991 Jan; 259(1):43-48
• CAS Registry Number:
  4-Chloro-m-phenylenediamine (CAS RN 5131-60-2) ; Nitro-p-phenylenediamine (CAS RN 5307-14-2) ; o-Phenylenediamine, dihydrochloride (CAS RN 615-28-1)
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b8ff981a617e990c709a5db5e9ab85c2d021bd41fd2d911172e9c37388da4a86d4abd5feb8c7193eeb84c311169ae102f63cd0198ad395a84c3510fa88c0e3eb
• Download URL:
  https://stacks.cdc.gov/view/cdc/205636/cdc_205636_DS1.pdf
• File Type:
  [PDF - 472.34 KB ]