Specific binding of (3H)methylscopolamine to glass fibre filters

Details

• Personal Author:
  Costa LG
• Description:
  The binding of tritium labeled methylscopolamine (MS) to glass fiber filters was investigated. The amount of binding was dependent on the buffer used for the incubation. When atropine was present, total binding was decreased 50 to 60%. Specific binding was noted in the presence of scopolamine, methylscopolamine, methylatropine, carbachol, oxotremorine, and acetylcholine. Total binding increased with increasing concentrations of MS. Specific binding to glass fiber filters was saturable. Presoaking the glass fiber filters in bovine serum albumin solution decreased total binding 45 to 60%, and specific binding 70 to 85%. Pretreatment of the filters with poly-L- lysine reduced total binding to glass fiber filters by more than 80% and virtually abolished specific binding. The author suggests that these findings indicate the presence on glass fiber filters of a binding site for MS. The author urges caution when MS is used as a muscarinic ligand and that measures to reduce filter binding or alternative separation procedures should be considered. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Occupational Health Safety
• Keywords:
  Analytical-chemistry Analytical-methods Chemical-analysis Filter-materials Filtration; Grants-other NIOSH-Grant NIOSH-Publication
• ISSN:
  0022-3573
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  239-240
• Volume:
  39
• Issue:
  3
• NIOSHTIC Number:
  nn:00232176
• Citation:
  J Pharm Pharmacol 1987 Mar; 39(3):239-240
• Contact Point Address:
  Environmental Health University of Washington Dept of Environ Hlth, SC-34 Seattle, WA 98195
• Federal Fiscal Year:
  1987
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  University of Washington, Seattle, Washington
• Peer Reviewed:
  False
• Start Date:
  19860929
• Source Full Name:
  Journal of Pharmacy and Pharmacology
• End Date:
  19900331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2f1e6ec3f7f6416c47b4ca68aae5f7f46ee7e62de7cfffd12f71da5f4ebf7cb379e722d6dfcba4113e2c7a81a800868dafd20f7d54abd971a351e23e3a8b48d4
• Download URL:
  https://stacks.cdc.gov/view/cdc/205620/cdc_205620_DS1.pdf
• File Type:
  [PDF - 172.98 KB ]