Silica-induced micronuclei and chromosomal aberrations in Chinese hamster lung (V79) and human lung (Hel 299) cells

Details

• Personal Author:
  Nagalakshmi R ; Nath J ; Ong T ; Whong Z
• Description:
  The clastogenic activity of two different sized distributions of silica (14808607) dusts were investigated in cultured Chinese- hamster lung fibroblasts (V79 cells) and human embryonic lung (Hel- 299) cells using two cytogenetic assay systems. The two forms of silica used were Min-U-Sil-5 with an average particle size of 5 micrometers, and Min-U-Sil-10, mostly in the 10 micrometer size range. The micronucleus assay and chromosomal aberration analysis were conducted on cells cultured with silica at 40 to 320 micrograms/square centimeter. Results of a cell cycle kinetics analysis of Hel-299 cells in the presence of cytochalasin-B indicate that with increases in culture time, there was a decrease in the proportion of mononucleated cells. The frequency of micronucleated V79 cells showed a dose related increase which was tied to an increase in silica concentration. At the highest concentration, a 13 fold increase was noted in total micronucleated binucleated cells over controls. In the Hel 299 cells there was an increase in frequency of micronucleated binucleated cells in both Min-U-Sil-5 and Min-U-Sil-10 treated cultures. Results of the chromosomal aberration studies indicated that the aberrations had not significantly increased by exposure to either Min-U-Sil-5 or Min-U- Sil-10 in either V79 or Hel-299 cells over the controls. There were, however, a few gaps observed for both cell lines and both types of silica tested. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Chromosomes Dust Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety Silicon Dioxide
• Keywords:
  Author Keywords: Silica Cell-cultures Chromosomal Aberration Chromosome-damage Genotoxic-effects Hel 299 Cells In-vitro-studies Lung-cells Micronucleus Mineral-dusts NIOSH-Author Respiratory-system-disorders Silica-dusts V79 Cells

  More +
• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  27-33
• Volume:
  335
• Issue:
  1
• NIOSHTIC Number:
  nn:00228675
• Citation:
  Mutat Res 1995 Aug; 335(1):27-33
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8ebadcd38e3e7f95872d4c39986ffdc6fb7be025e5b82aa4d803be3026b04112017e66f24604b727898f16ed5da24a506fbffffcb2b46848d2a8b5de48c5582a
• Download URL:
  https://stacks.cdc.gov/view/cdc/205596/cdc_205596_DS1.pdf
• File Type:
  [PDF - 498.51 KB ]