Reversible inhibition of intercellular communication among cardiac myocytes by halogenated hydrocarbons

Details

• Personal Author:
  Breitenstein, Michael J. ; Toraason M ; Wey HE
• Description:
  The effect of ten aliphatic halogenated hydrocarbons was examined in addition to halothane (151677) on intercellular communication in cultured heart cells harvested from 2 to 4 day old Sprague-Dawley- rats. The other ten compounds tested included methylene-chloride (75092), 1,2-dichloroethane (107062), 1,1,2-trichloroethane (79005), chloroform (67663), trichloroethylene (79016), 1,1,1-trichloroethane (71556), carbon-tetrachloride (56235), tetrachloroethylene (127184), 1,1,1,2-tetrachloroethane (630206), and pentachloroethane (76017). Myocytes were suffused with increasing concentrations of halocarbon added as a 0.2% solution of dimethyl-sulfoxide to M199 containing 1.8 millimolar calcium and 5% serum. Single cells were microinjected with the fluorescent probe Lucifer-yellow and dye coupling to adjacent cells was monitored. The findings demonstrated the inhibition of the intercellular communication in cultured heart cells by each of the 11 halogenated hydrocarbons. The immediate and completely reversible action of these chemicals on gap junctional communication paralleled their ability to sensitize the myocardium to catecholamine induced arrhythmias. The inhibition of the intercellular communication appeared to be largely dependent on the ability of these compounds to partition in the cell membrane. These findings supported the theory that the physicochemical properties of the halocarbons are a major factor in their acute effect on the heart. [Description provided by NIOSH]
• Subjects:
  Anesthesia Biopharmaceutics Cardiovascular Diseases Cardiovascular System Cytotoxins Hazardous Substances Hydrocarbons Laboratories Occupational Health Safety
• Keywords:
  Anesthetics Cardiovascular-system-disorders Cell-damage Chlorinated-hydrocarbons Cytotoxic-effects Laboratory-animals NIOSH-Author Pharmaceuticals Toxic-effects
• ISSN:
  0272-0590
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  59-65
• Volume:
  18
• Issue:
  1
• NIOSHTIC Number:
  nn:00204988
• Citation:
  Fundam Appl Toxicol 1992 Jan; 18(1):59-65
• CAS Registry Number:
  (±)-Halothane (CAS RN 151-67-7) ; 1,1,1,2-Tetrachloroethane (CAS RN 630-20-6) ; 1,1,1-Trichloroethane (CAS RN 71-55-6) ; 1,1,2-Trichloroethane (CAS RN 79-00-5) ; 1,2-Dichloroethane (CAS RN 107-06-2) ; Carbon tetrachloride (CAS RN 56-23-5) ; Chloroform (CAS RN 67-66-3) ; Dichloromethane (CAS RN 75-09-2) ; Pentachloroethane (CAS RN 76-01-7) ; Tetrachloroethylene (CAS RN 127-18-4) ; Trichloroethylene (CAS RN 79-01-6)
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  True
• Source Full Name:
  Fundamental and Applied Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3c4341f1743ab0ded0b500fed4b45872a167a3a61ce1ed01cb1119256288ed66f43599355b1e705ef6b4ea9993a632cd2ef45addc0e8791220eaa0b64a871373
• Download URL:
  https://stacks.cdc.gov/view/cdc/205540/cdc_205540_DS1.pdf
• File Type:
  [PDF - 3.94 MB ]