Depression of calcium dynamics in cardiac myocytes - a common mechanism of halogenated hydrocarbon anesthetics and solvents

Details

• Personal Author:
  Heinroth K ; Hoffmann P ; Plews P ; Richards D ; Toraason M
• Description:
  The ability of eight widely used halogenated hydrocarbons (HC) to depress calcium (Ca) ion dynamics in rat cardiomyocytes during excitation/contraction coupling was examined. Cardiac ventricular cells isolated from neonatal Sprague-Dawley-rats were exposed to dichloromethane (75092) (DCM), dichloroethane (1300216) (DCE), 1,1,2- trichloroethane (79005) (1,1,2-TCE), trichloroethylene (79016) (TRI), halothane (151677) (HAL), 1,1,1-trichloroethane (71556) (1,1,1-TCE), perchloroethylene (127184) (PER), or perchloroethane (67721) (PCE) in a suffusion chamber. Measurement of Ca2+ was performed by incubation of myocytes with fura-2 prior to HC exposure and analysis by phase contrast microscope and dual excitation spectrofluorometer. All HCs induced a reversible reduction of amplitude of electrically induced Ca+2 without significant effects on diastolic Ca+2. Overt damage to the ventricular myocytes was indicated by absence of fura-2 loss through leaky membranes. The accumulation of Ca+2 caused by depolarization with 90 nanomolar potassium-chloride (7447407) (KCl) was inhibited as well, but to a lesser degree. A moderate inhibition of electrically induced Ca+2 was observed in myocytes treated with inhibitors of Ca+2 release. Evidence that the tonic Ca+2 response after depolarization depended primarily on the sarcolemmal Ca+2 influx was observed. The HC appeared to affect the release and accumulation of Ca+2 by the sarcoplasmic reticulum. The authors conclude that changes of Ca+2 dynamics in cardiomyocytes may be a common mechanism of HC cardiotoxicity. [Description provided by NIOSH]
• Subjects:
  Anesthesia Cardiovascular System Cells, Cultured Fluorometry Hazardous Substances Hydrocarbons Occupational Health Safety
• Keywords:
  Anesthetics Author Keywords: Cardiomyocytes Ca2+ Cardiac-function Cell-biology Cell-cultures Chlorinated-hydrocarbons Fura-2 Halogenated Hydrocarbons In-vitro-study Ion-transport KCl Depolarization NIOSH-Author Sarcolemmal Membrane Sarcoplasmic Reticulum Toxic-effects Transients

  More +
• ISSN:
  0022-2828
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  26
• Issue:
  5
• NIOSHTIC Number:
  nn:00225766
• Citation:
  J Mol Cell Cardiol 1994 May; 26(5):579-589
• CAS Registry Number:
  (±)-Halothane (CAS RN 151-67-7) ; 1,1,1-Trichloroethane (CAS RN 71-55-6) ; 1,1,2-Trichloroethane (CAS RN 79-00-5) ; Dichloroethane (CAS RN 1300-21-6) ; Dichloromethane (CAS RN 75-09-2) ; Hexachloroethane (CAS RN 67-72-1) ; Potassium chloride (CAS RN 7447-40-7) ; Tetrachloroethylene (CAS RN 127-18-4) ; Trichloroethylene (CAS RN 79-01-6)
• Federal Fiscal Year:
  1994
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Molecular and Cellular Cardiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d76207444525af6d79c01f84457a32b2dd05d116b9a4b9cf00bc4bfc17509fdb9858c3bb85def153ce72d4b2f1b157c802e663e28b1bdb174a0146b8bbd9efa9
• Download URL:
  https://stacks.cdc.gov/view/cdc/204446/cdc_204446_DS1.pdf
• File Type:
  [PDF - 523.63 KB ]