Purification and characterization of cytochrome P-450 isozymes from phenobarbital-induced adult hen liver

Details

• Personal Author:
  Abou-Donia MB ; Gupta RP ; Lapadula DM
• Description:
  Cytochrome-P-450 isozymes from the livers of adult hens treated with phenobarbital (50066) were purified and characterized. Leghorn-hens were injected intraperitoneally with phenobarbital at 20mg/kg daily for 4 days; 24 hours after the last dose, they were killed, and liver microsomes were prepared. P-450 isozymes were separated by column chromatography. The isozymes were identified as P-450 PB-A and PB-B. Two highly specific P-450 antibodies were raised for both the isozymes, and these produced a single band with microsomes by immunoblotting. Each of the two purified P-450 isozymes had the same molecular weight, 54,000. However, each was easily distinguished using chromatographic behavior, spectral analysis, peptide mapping, tryptic peptide mapping, immunochemical properties, and amino terminal sequencing. There was no cross reactivity between the antibodies raised against P-450 PB-A and PB-B, and microsomal P450s of rat, mice, cat, or catfish species as determined by immunoblotting. [Description provided by NIOSH]
• Subjects:
  Digestive System Enzymes Immune System Laboratories Liver Nervous System Occupational Health Safety
• Keywords:
  Enzyme-activity Immunochemistry Laboratory-animals Liver-enzymes Microsomal-enzymes Neurotoxic-effects NIOSH-Grant NIOSH-Publication Oxidative-enzymes
• ISSN:
  0306-4492
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  163-176
• Volume:
  96
• Issue:
  1
• NIOSHTIC Number:
  nn:00232168
• Citation:
  Comp Biochem Physiol C Comp Pharmacol Toxicol 1990 Jan; 96(1):163-176
• Contact Point Address:
  Pharmacology Duke University Department of Pharmacology Durham, N C 27710
• CAS Registry Number:
  Phenobarbital (CAS RN 50-06-6)
• Federal Fiscal Year:
  1990
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2a2ed1f8d2381bf85b2b9f673eb216a399a492e2713bd4f0d2d6656bd5b5396b6a3423c2801ef9b7676fd05c3fa6a2c641ac749a349a7e1a9205100bcfe3307a
• Download URL:
  https://stacks.cdc.gov/view/cdc/205440/cdc_205440_DS1.pdf
• File Type:
  [PDF - 6.17 MB ]