Na+/K+-ATPase in rat brain and erythrocytes as a possible target and marker, respectively, for neurotoxicity: studies with chlordecone, organotins and mercury compounds

Details

• Personal Author:
  Costa LG ; Maier WE
• Description:
  The general aim of this study was to determine whether parameters of neurotransmission present in blood cells could serve as peripheral indicators of neurotoxicity, specifically the enzyme Na+/K+-ATPase which is important in regulating membrane function and neurotransmission. To validate the use of measurements of Na+/K+- ATPase in erythrocytes as a marker for the same enzyme in brain, the organochlorine pesticide chlordecone (143500), the organotins triethyltin (997502) and tributyltin (688733), mercuric-chloride (7487947) and methyl-mercury were chosen as chemical probes. Male Sprague-Dawley-rats were used in this study. All the compounds tested were shown to be potent in-vitro inhibitors of brain and erythrocyte Na+/K+-ATPase, yet administration of these compounds in- vivo at high doses produced symptoms of neurotoxicity without a corresponding inhibition of Na+/K+-ATPase. The reasons for the discrepancy between in-vitro inhibition and lack of detectable in- vivo inhibition is not always clear. In the case of triethyltin, mercuric-chloride, and methylmercury, the metal levels present in the brain following administration of neurotoxic doses were not high enough to inhibit Na+/K+-ATPase. No consistent patterns of parallel effects of enzyme activity in brain and erythrocytes was observed following in-vivo administration of various neurotoxic compounds. However, the recent finding of decreased erythrocyte Na+/K+-ATPase activities in workers chronically exposed to mercury (7439976) still suggests that chronic studies may be needed to infer or rule out conclusively a role for erythrocyte Na+/K+-ATPase as a useful peripheral marker for exposure to certain neurotoxicants. [Description provided by NIOSH]
• Subjects:
  Hematologic Tests Hydrocarbons Insecticides Laboratories Mercury Nervous System Occupational Health Poisoning Safety
• Keywords:
  Author Keywords: Na+/K+ -ATPase Biological-monitoring Blood-analysis Cell-function Chlordecone Chlorinated-hydrocarbons Laboratory-animals Mercury-poisoning Neurotoxic-effects Neurotoxicity NIOSH-Grant NIOSH-Publication Organo-mercury-compounds Organotins Peripheral Marker Pesticides

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• ISSN:
  0378-4274
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  175-188
• Volume:
  51
• Issue:
  2
• NIOSHTIC Number:
  nn:00195199
• Citation:
  Toxicol Lett 1990 Apr; 51(2):175-188
• Contact Point Address:
  Environmental Health University of Washington Dept of Environ Hlth, SC-34 Seattle, WA 98195
• CAS Registry Number:
  Chlordecone (CAS RN 143-50-0) ; Mercuric chloride (CAS RN 7487-94-7) ; Mercury (CAS RN 7439-97-6) ; Tributylstannane (CAS RN 61-Chloro-2-nitrobenzene (CAS RN 88-73-3)) ; Triethylstannane (CAS RN 997-50-2)
• Federal Fiscal Year:
  1990
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  University of Washington, Seattle, Washington
• Peer Reviewed:
  True
• Start Date:
  19860929
• Source Full Name:
  Toxicology Letters
• End Date:
  19900331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2dc4e86b3f06d076663b59a6c1993df9441684967151ded1c8b1a1686e437fab01ff25dfe3d04008bedbcc768f376421b4319cd75411e2be50fe51b9119960c5
• Download URL:
  https://stacks.cdc.gov/view/cdc/205170/cdc_205170_DS1.pdf
• File Type:
  [PDF - 937.03 KB ]