Laboratory methods for assessing human semen in epidemiologic studies: a consensus report

Details

• Personal Author:
  Chapin RE ; Clegg ED ; Davis RO ; Fourcroy JL ; Katz DF ; Rothmann SA ; Schrader, Steven M. ; Toth G ; Turner TW ; Zinaman M
• Description:
  Laboratory procedures for assessing human semen quality in epidemiological studies were discussed. The discussion was a summary of a meeting of andrologists who were currently using computer assisted semen analysis (CASA) techniques and endpoints in field and clinical studies in the spring of 1990. Guidelines for obtaining semen samples from donors were discussed. Factors that could affect semen quality such as abstinence time before obtaining a sample, use of masturbatory aids, and sample age were considered. Recommendations for handling semen samples were reviewed. Measuring sperm motility was discussed. Procedures for diluting semen samples were described. It was noted that establishing a standardized method for diluting semen samples is important because the existing CASA systems cannot accurately determine curvilinear velocity, straight line velocity, amplitude of lateral head displacement, or degree of linearity in samples containing sperm concentrations greater than 40 million per milliliter. Equipment recommendations for CASA systems were discussed. Proper machine settings for obtaining optimum semen data with the existing CASA systems were described. [Description provided by NIOSH]
• Subjects:
  Body Fluids Laboratories Occupational Health Safety Urogenital System
• Keywords:
  Body-fluids Humans Laboratory-equipment Laboratory-techniques NIOSH-Author Reproductive-system Sexual-reproduction Spermatozoa
• ISSN:
  0890-6238
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  California ; District of Columbia ; Maryland ; North Carolina ; Ohio ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 5 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  275-279
• Volume:
  6
• Issue:
  3
• NIOSHTIC Number:
  nn:00207733
• Citation:
  Reprod Toxicol 1992 May; 6(3):275-279
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  True
• Source Full Name:
  Reproductive Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0204cf7fc974490524f819d1e1a53a9070648a67c52efec70be7e855461247d910efa10855dd32b36f1042489b345cf91c78442212b5f73fbe64e25aa6df46a6
• Download URL:
  https://stacks.cdc.gov/view/cdc/205000/cdc_205000_DS1.pdf
• File Type:
  [PDF - 508.24 KB ]