Hydrocarbon solvents: effect on calcium mediated events in liposomes

Details

• Personal Author:
  Marion KJ ; Rabovsky J ; Resnick H
• Description:
  Experiments were conducted to determine the effects of hydrocarbon solvents on the calcium 2+ ion (Ca2+) induced turbidity in liposomes. Anionic liposomes consisting of a phospholipid bilayer containing dimyristoylphosphatidylcholine and 4% molar dicetylphosphate were prepared and the turbidity measured at 360 nanometers after 16 to 18 hours of incubation with test substances. Ca2+ had a maximal effect on the liposomes at concentrations between increase in turbidity. A reversal of Ca2+ induced turbidity of liposomes was seen with exposure to carbon-tetrachloride (56235) (CCl4), 1,1,1-trichloroethane (71556) (TCE), chloroform (67663) (CHCl3), cyclohexane (110827), hexane (110543) and benzene (71432). The data conformed to double reciprocal plot analysis. Apparent association constants were determined to be: 0.11mM(-1) for CCl4, 1) with 1mm Ca2+ for TCE. Based on these results the authors suggest that halogenated aliphatic solvents have a weak interaction with membrane binding sites. [Description provided by NIOSH]
• Subjects:
  Chemistry Halothane Lipids Occupational Health Safety Solvents
• Keywords:
  Author Keywords: Liposomes Calcium Chemical-analysis Chemical-kinetics Chemical-properties Halogenated-hydrocarbons NIOSH-Author Organic-solvents Phospholipids Plasma-membrane Solvents

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• ISSN:
  0009-3084
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  13-18
• Volume:
  37
• Issue:
  1
• NIOSHTIC Number:
  nn:00205290
• Citation:
  Chem Phys Lipids 1985 Apr; 37(1):13-18
• CAS Registry Number:
  1,1,1-Trichloroethane (CAS RN 71-55-6) ; Benzene (CAS RN 71-43-2) ; Carbon tetrachloride (CAS RN 56-23-5) ; Chloroform (CAS RN 67-66-3) ; Cyclohexane (CAS RN 110-82-7) ; Hexane (CAS RN 110-54-3)
• Federal Fiscal Year:
  1985
• Peer Reviewed:
  True
• Source Full Name:
  Chemistry and Physics of Lipids
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:18cfef011409ce02baf9b8cfceaec1ba8d8b4e33a8422782d2ff2dc037b8cd3c6507f178e0282166ed6148dbb4699976b2bc96a205372f929b41594b1f0004f9
• Download URL:
  https://stacks.cdc.gov/view/cdc/204863/cdc_204863_DS1.pdf
• File Type:
  [PDF - 472.58 KB ]