HOCl causes airway substance P hyperresponsiveness and neutral endopeptidase hypoactivity

Details

• Personal Author:
  Lang Z ; Murlas CG ; Murphy TP
• Description:
  Guinea-pig tracheal tissue was exposed to hypochlorous-acid (7790923) (HOCl) or hydrogen-peroxide (7722841) (H2O2) in an effort to determine whether there would be any effect on the responsiveness of airway muscle to acetylcholine (ACh), potassium-chloride (KCl), or substrate-P in the presence or absence of phosphoramidon. Tracheas were isolated from male Hartley-guinea-pigs, and used for airway contraction studies. Tissues were exposed to HOCl or H2O2 by perfusion through the airway lumen. A hyperresponsiveness to substance-P was noted in smooth muscle of mucosa intact guinea-pig airways which had been perfused with HOCl but not H2O2. No hyperreactivity was noted in response to ACh or KCl. Phosphoramidon pretreated, HOCl perfused rings behaved the same as Krebs solution exposed controls. An association between hyperresponsiveness and decreased neutral endopeptidase (NEP) activity was noted. In studying the catabolism of substance-P, it was noted that HOCl or phosphoramidon exposure decreased substance-P disappearance from the perfusates. The authors suggest that smooth muscle hyperresponsiveness to substance-P caused by the perfusion of airways with HOCl occurs through HOCl inactivation of mucosal NEP. [Description provided by NIOSH]
• Subjects:
  Enzymes Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Sense Organs
• Keywords:
  Asthma Author Keywords: Airway Hyperreactivity Bronchial Reactivity Bronchoconstriction Enkephalinase Enzyme Activity Guinea Pig Hypohalous Acids Laboratory Animals Lung Irritants Metalloendopeptidase Mucous Membranes Muscarinic Receptors NIOSH Grant NIOSH Publication Oxidant Injury Oxidizers Pulmonary System Disorders Respiratory Irritants Tachykinins Trachealis Muscle

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• ISSN:
  1040-0605
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  258
• Issue:
  6
• NIOSHTIC Number:
  nn:00232156
• Citation:
  Am J Physiol Lung Cell Mol Physiol 1990 Jun; 258(6):L361-L368
• Contact Point Address:
  Christopher G. Murlas, Internal Medicine Univ of Tennessee, Memphis 956 Court Avenue Memphis, TN 38163
• CAS Registry Number:
  Hydrogen peroxide (CAS RN 7722-84-1) ; Hypochlorous acid (CAS RN 7790-92-3)
• Federal Fiscal Year:
  1990
• NORA Priority Area:
  Pulmonary System Disorders
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19880301
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• End Date:
  19920229
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:02c7126480b62feb1f42dca84a7990ab75c7e17dc3820a93f668f8af97ce5667c3523322ee8845f4b9e814915bf48b11fd38b702e2ae12b44b1b770184e03cee
• Download URL:
  https://stacks.cdc.gov/view/cdc/204854/cdc_204854_DS1.pdf
• File Type:
  [PDF - 18.88 MB ]