Aerosolized neutral endopeptidase reverses ozone-induced airway hyperreactivity to substance P

Details

• Personal Author:
  Chodimella V ; Lang Z ; Murlas CG ; Williams GJ
• Description:
  Hartley-guinea-pigs were exposed to ozone (10028156) for 2 hours, and the effects on airway neutral endopeptidase (NEP) and reactivity to substance-P were studied. Intact, unanesthetized, spontaneously breathing male guinea-pigs were exposed to 3.0 parts per million ozone or to room air. Ozone exposure resulted in a substantial increase in airway reactivity to substance-P. Treatment with phosphoramidon caused a substantial increase in the substance-P reactivity of air exposed guinea-pigs. The values of NEP activities of tracheal homogenates from ozone exposed animals ranged from 16.2 to 20 nanomoles/minute milligram/protein and 22.7 to 27.5 in air exposed animals. In all animals tested, active NEP reduced substance-P reactivity after ozone exposure to preozone levels. Aerosolized phosphoramidon treatment following NEP reversed this finding. No significant influence on substance-P reactivity post ozone was demonstrated by heat inactivated NEP inhalation. A considerable decrease in substance-P reactivity produced by active NEP inhalation post ozone was noted. The authors suggest that an airway disorder associated with the loss of NEP activity can be reversed by inhaling an aerosol containing that enzyme. [Description provided by NIOSH]
• Subjects:
  Asthma Drug Therapy Enzymes Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Airway Resistance Bronchial Asthma Drug Therapy Enzyme Activity Inhalation Studies Laboratory Animals Lung Irritants NIOSH Grant NIOSH Publication Pulmonary System Disorders Respiratory System Disorders

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• ISSN:
  8750-7587
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  72
• Issue:
  3
• NIOSHTIC Number:
  nn:00206429
• Citation:
  J Appl Physiol 1992 Mar; 72(3):1133-1141
• Contact Point Address:
  Rush-Presbyterian St. Luke Medical Center, Department of Medicine (Pulmonary), 1653 W. Congress Parkway, Chicago, IL 60612
• CAS Registry Number:
  Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  1992
• NORA Priority Area:
  Pulmonary System Disorders
• Performing Organization:
  Rush-Presbyterian St. Luke Medical Center, Department of Medicine (Pulmonary), Chicago, IL 60612
• Peer Reviewed:
  True
• Start Date:
  19890301
• Source Full Name:
  Journal of Applied Physiology
• End Date:
  19920228
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f46da08297ad21c8d657dee92ac4e8f3abd612ff7b9e16dc39b93829f9694656f932468dde3dbd973823c1dcfbb40ddf38be24e1343f2eee9e03123ab64c2901
• Download URL:
  https://stacks.cdc.gov/view/cdc/204092/cdc_204092_DS1.pdf
• File Type:
  [PDF - 2.02 MB ]