Genotoxicity and genotoxic enhancing effect of tetrandrine in Salmonella typhimurium

Details

• Personal Author:
  Jiang X ; Lu H ; Ong T ; Stewart JD ; Whong Z
• Description:
  In this report the potential genotoxic and carcinogenic hazards of tetrandrine (518343) were studied using two genetic endpoints, gene mutation and DNA damage. The Salmonella/histidine reversion assay and the SOS/Umu test were used for measuring gene mutations and DNA damage, respectively. The modulating effect of tetrandrine on the genotoxicity of other mutagens and carcinogens was also examined. Tetrandrine was weakly mutagenic to Salmonella-typhimurium (TA-98) (approximately 2.7 times the control) but not with S-typhimurium (TA- 100). Metabolic activation was required for the mutagenic activity. Tetrandrine failed to induce an SOS response at doses up to 150 micrograms and 50 micrograms/treatment with and without metabolic activation, respectively. Tetrandrine produced a dose dependent enhancement of mutations induced by all the mutagens studied. The degree of enhancement ranged from approximately 80 percent with aflatoxin-B1 (1162658) to 200 percent with airborne particles. Tetrandrine increased the 2-aminoanthracene (613138) and trinitrofluorenone (129793) induced SOS responses by more than 300 percent. The authors conclude that tetrandrine was a weak promutagen inducing frameshift mutations and was a potent genotoxic enhancer. The mechanism for the genotoxic enhancement is not understood at present. However, it is suggested that the enhancement may result from an increase in error prone DNA repair. [Description provided by NIOSH]
• Subjects:
  Biological Assay Carcinogens DNA Mutagens Occupational Health Safety
• Keywords:
  Ames Assay Author Keywords: Tetrandrine Bioassays DNA-damage Genotoxic-effects Medical-treatment Mutagenicity NIOSH-Author Nucleic-acids Salmonella Typhimurium Silicosis SOS/Umu Test

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• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  237-244
• Volume:
  222
• Issue:
  3
• NIOSHTIC Number:
  nn:00187559
• Citation:
  Mutat Res 1989 Mar; 222(3):237-244
• CAS Registry Number:
  2-Aminoanthracene (CAS RN 613-13-8) ; Aflatoxin B1 (CAS RN 1162-65-8) ; Tetrandrine (CAS RN 518-34-3) ; Trinitrofluorenone (CAS RN 129-79-3)
• Federal Fiscal Year:
  1989
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:59ebd5855c7a523b143be4cfd922cd8dd0dc898bcad9c5dbf9ed47d03e236c161d412a2ff720b7dc980d0b9c749bba5eeac453f40ed06f4358a7de1598b22e0e
• Download URL:
  https://stacks.cdc.gov/view/cdc/204810/cdc_204810_DS1.pdf
• File Type:
  [PDF - 537.59 KB ]