Evaluation of the V79 cell metabolic co-operation assay as a screen in vitro for developmental toxicants

Details

• Personal Author:
  Bohrman JS ; Combes RD ; Krieg, Edward Jr. ; Langenbach R ; Toraason M ; Willington SE ; Zajac W
• Description:
  The utility of the V79 cell metabolic cooperation assay as an in- vitro screen for developmental toxicants was evaluated. Thirty- eight coded chemicals were tested for their effect on intercellular communication. A separate cytotoxicity assay was used to determine the concentration of each chemical that inhibited clonal expansion of V79 cells. Only seven of the 29 designated teratogens proved positive for inhibition of intercellular communication. Four teratogens and one nonteratogen inhibited intercellular communication at only a single concentration or at cytotoxic concentrations and were considered equivocal. The sensitivity of the assay for teratogens was therefore in the range of 24 to 38%. The overall accuracy for correctly identifying teratogens and nonteratogens was in the range of 42 to 50%. Specificity ranged from 89 to 100%. The authors conclude that chemicals which inhibit intercellular communication under the given conditions have a high probability of being teratogens. Reasons for reduced accuracy of the assay were considered. [Description provided by NIOSH]
• Subjects:
  Biological Assay Hazardous Substances Laboratories Occupational Health Safety
• Keywords:
  Analytical-methods Bioassays Cell-function Contract-200-84-2783 Contract-IA-85-14 In-vitro-study Laboratory-testing NIOSH-Author NIOSH-Contract NIOSH-Publication Screening-methods Toxic-materials

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• ISSN:
  0887-2333
• Document Type:
  Text
• Funding:
  Interagency Agreement ; Contract
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  165-174
• Volume:
  6
• Issue:
  2
• NIOSHTIC Number:
  nn:00209919
• Citation:
  Toxicol In Vitro 1992 Mar; 6(2):165-174
• Contact Point Address:
  M. Torrason, Cellular Toxicology Section C23, DBBS, NIOSH, CDC, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology in Vitro
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f58f5aa6071c18ee6da971f311d8505a934d4b806f374091a2ebf585022d3cecdcdde3bfd04c5f769e13ad5491ff7a69b8f5cd0d094b4ba383fd6145b53c8760
• Download URL:
  https://stacks.cdc.gov/view/cdc/204703/cdc_204703_DS1.pdf
• File Type:
  [PDF - 827.07 KB ]