Enhanced IL-1 beta and tumor necrosis factor-alpha release and messenger RNA expression in macrophages from idiopathic pulmonary fibrosis or after asbestos exposure

Details

• Personal Author:
  Guillemin B ; Lee TC ; Rom, William N. ; Yu C ; Zhang Y
• Description:
  Bronchoalveolar lavage (BAL) was performed on patients with idiopathic pulmonary fibrosis (IPF) and on individuals who had asbestos (1332214) exposure with radiographic evidence of parenchymal fibrosis to obtain alveolar macrophages (AM) for analysis of IL-1beta and TNF-alpha protein release and gene expression. BAL recovered significantly more cells in asbestos exposed and IPF patients than in normal controls. Both patient groups released significantly more TNF-alpha than controls. Only the asbestos exposed subjects released significantly more IL-1beta than controls. A significant correlation was found between the number of neutrophils per milliliter of BAL fluid recovered and the spontaneous release of TNF-alpha for both groups combined. AM lavaged from patients expressed increased mRNA levels for both IL- 1beta and TNF-alpha. The increased production of IL-1beta and TNF- alpha was caused by enhanced expression of the mRNA for the cytokines. The authors note that the cytokines have the potential to influence the remodeling and fibrosis observed in the lower respiratory tract in IPF and asbestosis. [Description provided by NIOSH]
• Subjects:
  Asbestos Cytotoxins Inorganic Chemicals Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Alveolar-cells Asbestos-fibers Cell-damage Cytotoxic-effects Humans Lung-fibrosis Lung-irritants NIOSH-Cooperative-Agreement NIOSH-Publication Respiratory-system-disorders
• ISSN:
  0022-1767
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  150
• Issue:
  9
• NIOSHTIC Number:
  nn:00227514
• Citation:
  J Immunol 1993 May; 150(9):4188-4196
• Contact Point Address:
  Dr. W. N. Rom, Division of Pulmonary and Critlcal Care Medicine, Bellevue Hospital Center Rm 7N24, New York University Medical Center, 550 First Avenue, New York, NY 10016
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4)
• Federal Fiscal Year:
  1993
• NORA Priority Area:
  Pulmonary System Disorders
• Peer Reviewed:
  True
• Source Full Name:
  The Journal of Immunology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ef08758cf040ac3dee4f2c962b68603843842c55f620e7813598a1e34bd1ffca53e041bb7d2e964ebd942e36cfc2ed17d842c0b67c82218bb9700a3fffd98cc4
• Download URL:
  https://stacks.cdc.gov/view/cdc/204609/cdc_204609_DS1.pdf
• File Type:
  [PDF - 2.95 MB ]