Expression and Activity of Urokinase and Its Receptor in Endothelial Cells Exposed to Asbestos

Details

• Personal Author:
  Barchowsky A ; Fava RA ; Hunt JA ; Krieser RJ ; Treadwell MD
• Description:
  Endothelial cells were exposed to chrysotile (12001295), crocidolite (12001284), or refractory-ceramic-fiber-1 (RCF-1) in an effort to determine whether asbestos induced endothelial cell activation is associated with altered proteolysis and expression of urokinase-like- plasminogen-activator (uPA) and urokinase-like-plasminogen-activator- receptor (uPAR). Second or third passage porcine aortic endothelial cells grown to confluence were incubated with the fibers for up to 24 hours. In-situ zymography for uPA activity in exposed cultures demonstrated that these fibers produce localized increases in proteolysis. Within 8 hours, exposure to chrysotile or crocidolite increased uPA expression. The increases in proteolysis correlate well with increases in steady state uPA mRNA levels and with increased levels of uPAR protein. In contrast, rRCF-1 has a lower fibrogenic potential than asbestos and it failed to elicit endothelial cell activation or affect uPA/uPAR. The authors suggest that asbestos fibers directly activate vascular endothelial cells to express proteins that facilitate tissue remodeling. [Description provided by NIOSH]
• Subjects:
  Asbestos Cytotoxins Dust Inorganic Chemicals Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Asbestos-fibers Cell-damage Cytotoxic-effects Fibrous-dusts Lung-irritants Mammalian-cells NIOSH-Grant Pulmonary-system-disorders
• Series:
  Grant Final Reports
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Final Grant Report
• Place as Subject:
  New Hampshire ; OSHA Region 1 ; Vermont
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OEP - Office of Extramural Programs
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-28
• NIOSHTIC Number:
  nn:00238185
• NTIS Accession Number:
  PB97-206304
• Citation:
  Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire and Department of Medicine, Veterans Hospital, White River Junction, Vermont 1996 Jan; :1-28
• Contact Point Address:
  Pharmacology and Toxicology Dartmouth Medical School Remsen 7650 Hanover, NH 03755-3835
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4) ; Chrysotile (CAS RN 12001-29-5) ; Crocidolite asbestos (CAS RN 12001-28-4)
• Federal Fiscal Year:
  1996
• NORA Priority Area:
  Pulmonary System Disorders
• Performing Organization:
  Dartmouth College, Hanover, New Hampshire
• Peer Reviewed:
  False
• Start Date:
  19940930
• Source Full Name:
  Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire and Department of Medicine, Veterans Hospital, White River Junction, Vermont
• End Date:
  19960929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a845ddd9782c2797d90274d9dfa3204ea7fc5cfd4b0498f29369961df5c3539de59bbd8e2fe198c0bab12befcd8187313502e017aa3427d2ff380b5664b46a34
• Download URL:
  https://stacks.cdc.gov/view/cdc/217913/cdc_217913_DS1.pdf
• File Type:
  [PDF - 931.76 KB ]