Effects of methoxychlor on the reproductive system of the adult female mouse: 2. Ultrastructural observations

Details

• Personal Author:
  Martinez EM ; Swartz WJ
• Description:
  The ultrastructure of different cellular compartments of the adult ovary in mice exposed to the pesticide methoxychlor (72435) (MXC), was investigated in order to assess subcellular toxic effects and their relevance, if any, to lowered reproductive capacity. Virgin female adult CD-1-mice were treated with estradiol-17beta (50282) (E- 17b) or MXC at a dose approximately equivalent to 200 mg/kg. Controls were treated with sesame-oil. Five consecutive days of exposure were followed by 2 days of no treatment to mimic a regular 5 day work week. At 24 hours after the last exposure, animals were killed and ovaries were processed for electron microscopy. Ultrastructural changes were evaluated, with atresia being assessed according to the method of Gondos. Steroid secreting interstitial cells of the ovaries in sesame-oil treated controls had little granular endoplasmic reticulum (RER) and extensive smooth endoplasmic reticulum (SER). The amount of lipid present in these cells, of both MXC and E-17b treated mice, far exceeded that in controls. Theca cells which also produce steroids, contained excessive RER and minimal SER, indicating a reduction in steroidogenesis, in both E-17b and MXC treated animals, confirming the estrogen like effects of MXC. The authors conclude that the ovary of the adult nonpregnant female is the target organ for MXC effects, the result of which appears to be a blockage of steroid biosynthesis. [Description provided by NIOSH]
• Subjects:
  Chlorine Compounds Cytotoxins Estrogens Insecticides Laboratories Occupational Health Safety Urogenital Diseases Urogenital System
• Keywords:
  Chlorinated-hydrocarbon-pesticides Cytotoxicity Estrogenic-hormones Histopathology Laboratory-animals NIOSH-Grant NIOSH-Publication Organo-chlorine-compounds Reproductive-hazards Reproductive-system-disorders
• ISSN:
  0890-6238
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Louisiana ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  93-98
• Volume:
  6
• Issue:
  1
• NIOSHTIC Number:
  nn:00205805
• Citation:
  Reprod Toxicol 1992 Jan; 6(1):93-98
• Contact Point Address:
  Anatomy Louisiana State University 1542 Tulane Avenue New Orleans, LA 70112
• CAS Registry Number:
  Estradiol (CAS RN 50-28-2) ; Methoxychlor (CAS RN 72-43-5)
• Federal Fiscal Year:
  1992
• Performing Organization:
  Louisiana State University, School of Medicine, Department of Anatomy, New Orleans, Louisiana
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Reproductive Toxicology
• End Date:
  19980831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3988e1538696af298bed5aff37a3c9a136cfafc704cfb6b2f66949f32ff5e93dbf6d0dec6a92bcccedfc404bb67e5fd9034277b54ae6cb7772fde926adef9ea4
• Download URL:
  https://stacks.cdc.gov/view/cdc/204559/cdc_204559_DS1.pdf
• File Type:
  [PDF - 3.31 MB ]