Effects of ammonium metavanadate treatment upon macrophage glutathione redox cycle activity, superoxide production, and intracellular glutathione status

Details

• Personal Author:
  Cohen MD ; Wei CI
• Description:
  B6C3F1-mice were administered ammonium-metavanadate (7803556) at 10mg/kg, or 2.5mg/kg as vanadium (7440622) intraperitoneally every 3 days for 6 weeks, and the effects on three enzymes involved in energy production for phagocytosis and for the removal of peroxides, as well as the production of superoxide anion were studied. An attempt was made to determine whether vanadate pretreatment could contribute to the observed depressed phagocyte responses and Listeria killing by inhibiting enzymes related to the respiratory burst in peritoneal macrophages (PEM). These enzymes included glucose-6-phosphate-dehydrogenase, glutathione-reductase and glutathione-peroxidase. The results clearly showed that intraperitoneal exposure of mice to vanadium for 6 weeks could affect the three cytoplasmic enzymes which are crucial for PEM function, as well as the level of substrate required for their proper activity. The authors suggest that their 6 week exposure period may serve as a model for analyzing the effects of vanadium on macrophage function after long term exposure. [Description provided by NIOSH]
• Subjects:
  Enzymes Hazardous Substances Laboratories Occupational Health Oxidants Safety Vanadium Compounds
• Keywords:
  Author Keywords: Mice Enzyme-activity Enzyme Activities Grants-other Laboratory-animals NIOSH-Grant NIOSH-Publication Oxidative-metabolism Protein Toxic-effects Vanadium-compounds

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• ISSN:
  0741-5400
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Florida ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  122-129
• Volume:
  44
• Issue:
  2
• NIOSHTIC Number:
  nn:00232394
• Citation:
  J Leukoc Biol 1988 Aug; 44(2):122-129
• Contact Point Address:
  Cheng-i Wei, Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611
• CAS Registry Number:
  Ammonium vanadium oxide ((NH4)VO3) (CAS RN 7803-55-6) ; Vanadium (CAS RN 7440-62-2)
• Federal Fiscal Year:
  1988
• NORA Priority Area:
  Grants Other
• Performing Organization:
  University of Florida, Gainesville, Florida
• Peer Reviewed:
  True
• Start Date:
  19880401
• Source Full Name:
  Journal of Leukocyte Biology
• End Date:
  19910331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c03eb0e54f459194d2ca0f544c1ef7699ee09bac02c72fc609dc34febc2531942d4e665398f6177cf1b8abb33a5d2a2e3f6c5122d8645e8593ef4c08390d3aa2
• Download URL:
  https://stacks.cdc.gov/view/cdc/204548/cdc_204548_DS1.pdf
• File Type:
  [PDF - 3.43 MB ]