Effect of ammonium metavanadate on the mouse peritoneal macrophage lysosomal enzymes

Details

• Personal Author:
  Vaddi K ; Wei C
• Description:
  The effect of vanadate on the activities and kinetics of select lysosomal enzymes was examined. The possibility that this was a contributing mechanism to the depression of the intracellular killing ability in peritoneal macrophages (PEM) from vanadate treated mice was considered. Ammonium-metavanadate (7803556) at 2.5 or 10mg/kg vanadium (7440622), ammonium-chloride (12125029), or sodium-phosphate buffer were used to inject intraperitoneally female B6C3F1-mice every third day for 6 weeks. Acid-phosphatase activity for the 2.5 and 10mg/kg vanadium groups was depressed by 22.8 and 44%, respectively. Vanadium treatment did not cause any significant effect on the other three enzymes. Ammonium-metavanadate caused similar effects as vanadium. Ammonium-chloride and vanadium treatments caused an enhancement of extracellular secretion of beta- glucuronidase and lysozyme from PEM. This could be attributed to the presence of ammonium ion. The findings suggested that the decreased bactericidal activity in the PEM of vanadate treated mice was not due to direct inhibition of either activity or synthesis of beta-glucuronidase, N-acetyl-beta-D-glucosaminidase or lysozyme. The authors suggest that the decrease in acid-phosphatase activity may contribute to the diminished intracellular killing ability of PEM. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Enzymes Laboratories Occupational Health Safety Vanadium Compounds
• Keywords:
  Cell-damage Cell-function Chemical-kinetics Cytotoxic-effects Enzyme-activity Laboratory-animals NIOSH-Grant NIOSH-Publication Vanadium-compounds
• ISSN:
  0098-4108
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Florida ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  65-78
• Volume:
  33
• Issue:
  1
• NIOSHTIC Number:
  nn:00200973
• Citation:
  J Toxicol Environ Health 1991 May; 33(1):65-78
• Contact Point Address:
  Food Science & Human Nutrition University of Florida Gainesville, FL 32611 Gainesville, FL 32611
• CAS Registry Number:
  Ammonium chloride (CAS RN 12125-02-9) ; Ammonium vanadium oxide ((NH4)VO3) (CAS RN 7803-55-6) ; Vanadium (CAS RN 7440-62-2)
• Federal Fiscal Year:
  1991
• Performing Organization:
  University of Florida, Gainesville, Florida
• Peer Reviewed:
  True
• Start Date:
  19880401
• Source Full Name:
  Journal of Toxicology and Environmental Health
• End Date:
  19910331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:05f5af5933424cb3d9fb152d41c78bb2106c652d187608796ffa90eddb5097a0aacf4ed9a961faad1c238f25899ec8b8999aa14a803ca1472c9ffce12707e267
• Download URL:
  https://stacks.cdc.gov/view/cdc/204527/cdc_204527_DS1.pdf
• File Type:
  [PDF - 944.71 KB ]