Effect of phorbol and bryostatin I on chondrogenic expression of chick limb bud, in vitro

Details

• Personal Author:
  Garrison JC ; Pettit GR ; Uyeki EM
• Description:
  The effect of phorbol-12-myristate-13-acetate (16561298) (PMA) on chondrogenesis was studied in chick limb bud cells. The effect of bryostatin-I (83314016) was examined for comparison purposes. Limb bud cells harvested from stage 23 or 24 White-Leghorn-chick embryos were incubated with up to 10(-6) molar (M) PMA or 10(-7)M bryostatin- I alone or in combination for up to 96 hours. The effects on cell proliferation were determined using a Coulter channelyzer. The effects on chondrogenesis were assessed by determining the extent of cartilage nodule formation and Alcian-blue dye staining for chondroitin-sulfate. Four days' exposure to 10(-7)M or higher PMA caused a significant decrease in cartilage nodule formation and the amount of Alcian-blue staining. No effects on cell proliferation were observed. When examined according to incubation time, the greatest inhibition of chondrogenesis occurred after 49 to 96 hours of PMA treatment. Bryostatin-I had a slight stimulatory effect on cartilage nodule dye formation and Alcian-blue staining. Coincubation of 10(-7)M bryostatin-I with 10(-7)M PMA abolished the inhibitory effect of PMA on chondrogenesis. The authors conclude that the inhibitory effect of PMA on chondrogenesis is due to a suppression of chondrocyte phenotypic expression rather than cell commitment to chondrocytic expression. Since both PMA and bryostatin-I are protein-C-kinase activators, the mechanism of PMA induced chondrogenesis inhibition must involve processes other than protein-C-kinase activation. [Description provided by NIOSH]
• Subjects:
  Enzymes Growth Laboratories Occupational Health Safety
• Keywords:
  Biokinetics Dose-response Enzyme-activity Grants-other Growth-inhibition Histochemical-analysis In-vitro-studies Laboratory-animals NIOSH-Grant NIOSH-Publication
• ISSN:
  0024-3205
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Kansas ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  41
• Issue:
  17
• NIOSHTIC Number:
  nn:00213644
• Citation:
  Life Sci 1987 Oct; 41(17):2055-2061
• Contact Point Address:
  Dr. Edwin M. Uyeki, Dept. of Pharmacology, Toxicology and Therapeutics, The University of Kansas Medical Center, 39th and Rainbow Blvd., Kansas City, Kansas 66103
• CAS Registry Number:
  Bryostatin 1 (CAS RN 83314-01-6) ; Phorbol 12-myristate 13-acetate (CAS RN 16561-29-8)
• Federal Fiscal Year:
  1988
• NORA Priority Area:
  Grants Other
• Performing Organization:
  University of Kansas Col Hlth Sci & Hosp, Kansas City, Kansas
• Peer Reviewed:
  True
• Start Date:
  19830930
• Source Full Name:
  Life Sciences
• End Date:
  19940831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:691b7b6a0fe0dde1a9b6cddf3bf9d2426ae89a5a712ff094bff81e182d36cea772412c0db633fb750d4680292ac000fb47b09f073cd85c2c35297d791c59198c
• Download URL:
  https://stacks.cdc.gov/view/cdc/204534/cdc_204534_DS1.pdf
• File Type:
  [PDF - 389.27 KB ]