Association of cytochrome b5 and cytochrome P-450 reductase with cytochrome P-450 in the membrane of reconstituted vesicles

Details

• Personal Author:
  Bosterling B ; Trudell JR
• Description:
  The interaction of cytochrome-P-450 and NADPH-cytochrome-P-450- reductase was studied in vesicle reconstituted systems. Cytochrome-P- 450 and NADPH-cytochrome-P-450-reductase were derived from livers of rabbits pretreated with phenobarbital. The cytochrome-P-450 and NADPH-cytochrome-p-450 were reconstituted into phospholipid vesicles. The vesicle systems contained sufficient phospholipid that no protein/protein interaction would be induced solely to prevent exposure of hydrophobic protein surfaces to the aqueous environment. Cytochrome-b5 was included in order to study a more complete NADPH dependent electron chain. The bilayer of these vesicles was composed of a 2:1 mixture of phosphatidylcholine and phosphatidylethanolamine which provided functional and structural similarity to microsomes. Complex formation in the membrane was studied by magnetic CD. Enzymatic measurements were used to study the stimulatory role of cytochrome-b5 and to obtain information on the electrostatic nature of the protein/protein interactions. The data supported a model of specific reversible association reactions in a two dimensional phospholipid bilayer in which electrostatic interactions are involved. The authors conclude that there is a requirement for at least one charge pairing for electron transfer from reductase to cytochrome-P-450. [Description provided by NIOSH]
• Subjects:
  Digestive System Enzymes Liver Occupational Health Safety
• Keywords:
  Enzyme-activity Grants-other In-vitro-studies Liver-enzymes NIOSH-Grant NIOSH-Publication Protein-chemistry
• ISSN:
  0021-9258
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  257
• Issue:
  9
• NIOSHTIC Number:
  nn:00187018
• Citation:
  J Biol Chem 1982 May; 257(9):4783-4787
• Contact Point Address:
  Anesthesia Stanford University Department of Anesthesia Stanford, Calif 94305
• Federal Fiscal Year:
  1982
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Journal of Biological Chemistry
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1fed0f8f3ad68b05637e40fcccfa99f7153ca11755c949e44d9ed563d9a92f4e9355b7f12a793ff08b5cca9494ff4633fa5723948086b66a6875470c59b8edfb
• Download URL:
  https://stacks.cdc.gov/view/cdc/204206/cdc_204206_DS1.pdf
• File Type:
  [PDF - 569.33 KB ]