Development of a fibroblast proliferation bioassay to detect mediators of pulmonary fibrosis

Details

• Personal Author:
  Blackford J ; Bryner K ; Castranova, Vincent ; Dey R ; Reist R ; Vrana K ; Wearden P
• Description:
  The effects of platelet derived growth factor (PDGF) and 2% rat serum, as a source of competence factor, on mitogenesis was investigated. Sprague-Dawley-rat lung fibroblasts were isolated and human fetal lung fibroblasts obtained from the American Tissue Culture Collection (ATCC153). After plating, cells were rested for 48 hours (hr) in plasma free media, then exposed to 150 nanograms/milliliter (ng/ml) PDGF and 2% rat plasma, separately or in combination for 6hr. Incorporation of tritiated thymidine during a 24hr labeling period in plasma free medium following the test treatments provided a measurement of mitogenesis. Positive controls were 10% and 20% fetal calf serum for rat and human fibroblasts, respectively. Serum free medium served as the negative control. Significant mitogenesis did not occur in cells treated with PDGF or 2% rat serum alone. Cells treated with a combination of PDGF and 2% rat serum incorporated significantly more thymidine than the positive controls, indicating activation of mitogenesis. A dose response curve demonstrated the sensitivity of the competence stimulating activity assay to a lower limit of 25ng/ml PDGF. The authors conclude that the described assay for fibrogenic factor production represents an improvement over previously published methods. [Description provided by NIOSH]
• Subjects:
  Biological Assay Dust Growth Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Bioassays Growth-factors In-vitro-study Lung-cells Lung-fibrosis Mammalian-cells Mitosis Pulmonary-disorders Quantitative-analysis Respirable-dust Respiratory-system-disorders

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• ISBN:
  9780873350983
• Publisher:
  Society for Mining, Metallurgy, and Exploration, Inc.
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  Colorado ; OSHA Region 3 ; OSHA Region 8 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  179-181
• NIOSHTIC Number:
  nn:00232442
• Citation:
  Respirable dust in the mineral industries, proceedings of the 3rd symposium on respirable dust in the mineral industries, October 17-19, 1990, Pittsburgh, Pennsylvania. Frantz RL, Ramani RV, eds. Littleton, CO: Society for Mining, Metallurgy, and Exploration, Inc., 1991 Jan; :179-181
• Editor(s):
  Frantz RL; Ramani RV
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  False
• Source Full Name:
  Respirable dust in the mineral industries, proceedings of the 3rd symposium on respirable dust in the mineral industries, October 17-19, 1990, Pittsburgh, Pennsylvania
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c5fac62ba8af02ad0ef65dbb43eca3b85adb65fca1a34e2e44ade83d7b461e5a6e856803f2ad3a5ae2d29c3a874b9b1f2473b4ee75c876f8b6c49e9e874d7a35
• Download URL:
  https://stacks.cdc.gov/view/cdc/204477/cdc_204477_DS1.pdf
• File Type:
  [PDF - 2.17 MB ]