Development and optimization of a supercritical fluid extraction method for the analysis of aflatoxin b1 in grain dust

Details

• Personal Author:
  Selim MI ; Tsuei H
• Description:
  A new technique for the extraction of grain dust aflatoxin-B1 (1162658) using supercritical fluid extraction (SFE) was developed and optimum conditions were derived. Grain dust samples were collected from farms in Iowa during the winter of 1990/1991. The samples were gathered from dust settled on floors and equipment in the loading and unloading areas, feed grinding areas, and storage bins. Most dust was from corn. About 2 grams (g) of a dust sample were mixed with celite and water, shaken with chloroform, and the extract was filtered. The residue was collected in methylene- chloride, evaporated to dryness, and redissolved in methanol. The SFE procedure used liquid carbon-dioxide (CO2) at 40 degrees-C with acetonitrile added. Static extraction was carried out for 15 minutes. A high performance liquid chromatography (HPLC) system with ultraviolet detection was calibrated using 2 nanograms (ng), 4ng, and 8ng of aflatoxin-B1. Results for 18 grain dust samples extracted using SFE and classical liquid/liquid extraction were presented. SFE detected aflatoxin-B1 in 14 samples, while the classical method did so for seven. Optimization for temperature, pressure, and CO2 volume was carried out. The method detection limit was calculated to be 1ng/gram for dust samples between 0.1 to 0.2 gram, using offline HPLC. The authors conclude that SFE is faster, more sensitive, and more efficient than classical liquid extraction, and it eliminates the use of hazardous organic solvents. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Carcinogens Chromatography Dust Fungi Mycoses Occupational Health Particulate Matter Safety
• Keywords:
  Airborne-dusts Chromatographic-analysis Dust-extraction Grain-dusts Grants-other Mycotoxins NIOSH-Grant NIOSH-Publication Sample-preparation
• ISSN:
  0002-8894
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Iowa ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  135-141
• Volume:
  54
• Issue:
  4
• NIOSHTIC Number:
  nn:00213523
• Citation:
  Am Ind Hyg Assoc J 1993 Apr; 54(4):135-141
• Contact Point Address:
  Preventive Medicine and Environmental Health, University of Iowa, 137 Amrf Oakdale Campus, Iowa City, IA 52242
• CAS Registry Number:
  Aflatoxin B1 (CAS RN 1162-65-8)
• Federal Fiscal Year:
  1993
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  University of Iowa, Iowa City, Iowa
• Peer Reviewed:
  True
• Start Date:
  19910501
• Source Full Name:
  American Industrial Hygiene Association Journal
• End Date:
  19980531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0b79ce7412dd09ce566392819cb1c56075e919285e25b1fd60f3d6e1ee1f1c182e7887ec12d7c4bef647f5cac2775304a98d52008ddf240766c5acd35f79a3b6
• Download URL:
  https://stacks.cdc.gov/view/cdc/204473/cdc_204473_DS1.pdf
• File Type:
  [PDF - 766.17 KB ]