Comparative metabolism of bis(2-methoxyethyl)ether in isolated rat hepatocytes and in the intact rat: effects of ethanol on in vitro metabolism

Details

• Personal Author:
  Begley KB ; Cheever KL ; DeBord, D. Gayle ; Richards, Donald E. ; Savage RE Jr. ; Tirmenstein MA ; Weigel WW
• Description:
  The effect of pretreatment of Sprague-Dawley-rats with ethanol (64175) on the metabolism of bis(2-methoxyethyl)ether (111966) (diglyme) was examined in-vivo and in isolated hepatocytes. The principal metabolite from hepatocytes and in the urine was (2- methoxyethoxy)acetic-acid (16024569) (MEAA). Other prominent metabolites included 2-(2-methoxyethoxy)ethanol (111773), methoxyacetic-acid (625456) (MAA), 2-methoxyethanol (109864) and diglycolic-acid (110996). The results of an in-vivo/in-vitro comparative metabolism study indicated that isolated rat hepatocytes in monolayer culture can be used to predict the urinary metabolites of diglyme. The urinary metabolite MEAA would best serve as a short term indicator of exposure to diglyme. The in-vitro metabolism of diglyme in hepatocytes isolated from rats pretreated with ethanol was increased over that in hepatocytes from untreated rats. Of particular significance was the induction of hepatic enzymes by ethanol which resulted in the conversion of increased amounts of diglyme to MAA, the metabolite associated with the reproductive and developmental toxicity of diglyme. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Digestive System Digestive System Diseases Energy Metabolism Ether Laboratories Liver Occupational Health Safety Solvents
• Keywords:
  Alcohols Cytotoxic-effects Ethers Laboratory-animals Liver-cells Liver-damage Metabolic-study NIOSH-Author Organic-solvents Solvent-vapors
• ISSN:
  0340-5761
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  67
• Issue:
  8
• NIOSHTIC Number:
  nn:00217475
• Citation:
  Arch Toxicol 1993 Sep; 67(8):531-537
• CAS Registry Number:
  (2-Methoxyethoxy)acetic acid (CAS RN 16024-56-9) ; 2-Methoxyethanol (CAS RN 109-86-4) ; Diethylene glycol monomethyl ether (CAS RN 111-77-3) ; Diglycolic acid (CAS RN 110-99-6) ; Diglyme (CAS RN 111-96-6) ; Ethanol (CAS RN 64-17-5) ; Methoxyacetic acid (CAS RN 625-45-6)
• Federal Fiscal Year:
  1993
• Peer Reviewed:
  True
• Source Full Name:
  Archives of Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eeda2777ce0ff438543aec4bae8c5a3fe425f682d33755e077904b6c33026e3918fcc40affb8aac42fef5dc5141859d6fa1a34961ae24abc45b352885b2fd63e
• Download URL:
  https://stacks.cdc.gov/view/cdc/204364/cdc_204364_DS1.pdf
• File Type:
  [PDF - 793.21 KB ]