Analysis of urine to monitor exposures to benzidine, o-dianisidine, o-tolidine, and 4,4'-methylenedianiline

Details

• Personal Author:
  Neumeister, Charles E.
• Description:
  A sampling and analytical procedure was developed for measuring urine concentrations of benzidine (92875), ortho-dianisidine (119904), ortho-tolidine (119937), and 4,4'-methylenedianiline (101779) for use in industrial hygiene investigations. After hydrolysis of urine metabolites, the free diamines were isolated using a C18 solid sorbent. The free diamines were eluted, concentrated, and injected into a high performance liquid chromatography system. Using a C18 column with a mobile phase of acetonitrile and water, separation was achieved. By monitoring the ultraviolet absorbance at 280 or 245 nanometers and the electrochemical response, identification and quantitation were achieved. The limit of detection for these aromatic diamines was less than 2 micrograms/liter using ultraviolet detection while the limit of quantitation was less than 6 micrograms/liter. For electrochemical detection the limit of detection was less than 0.3 micrograms/liter and the limit of quantitation was less than 0.9 micrograms/liter. Recoveries ranged from 87 to 102% at 2, 10, and 20 micrograms/liter levels. [Description provided by NIOSH]
• Subjects:
  Aniline Compounds Benzidines Chemistry Chemistry, Analytic Occupational Health Safety Toluidines Urine
• Keywords:
  Analytical-chemistry Analytical-methods Anilines Biological-monitoring Chemical-analysis NIOSH-Author Quantitative-analysis Urinalysis
• ISSN:
  1047-322X
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  6
• Issue:
  11
• NIOSHTIC Number:
  nn:00203126
• Citation:
  Appl Occup Environ Hyg 1991 Nov; 6(11):953-958
• CAS Registry Number:
  4,4'-Diaminodiphenyl (CAS RN 92-87-5) ; 4,4'-Diaminodiphenylmethane (CAS RN 101-77-9) ; Dianisidine (CAS RN 119-90-4) ; o-Tolidine (CAS RN 119-93-7)
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  True
• Source Full Name:
  Applied Occupational and Environmental Hygiene
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4abbe6c233f21594ed097bb6a6a5c5a92a588fce06788631f1cca7f5840079c4449c24d46c73087a1eb4053010a38a38e9111117f4e9e7eb2bf4f6831988b17e
• Download URL:
  https://stacks.cdc.gov/view/cdc/204166/cdc_204166_DS1.pdf
• File Type:
  [PDF - 781.91 KB ]