The effects of dipalmitoyl lecithin coated fresh quartz on superoxide release from alveolar macrophages in vitro

Details

• Personal Author:
  Cilento EV ; Georgellis GB
• Description:
  The hypothesis that freshly-sheared quartz makes it more reactive in lung tissue than aged quartz was tested by its ability to stimulate superoxide anion (O2-) release from single adherent pulmonary alveolar macrophages (PAM) . Fresh quartz was prepared by grinding for 30 min and then used within 2 hr, while stale quartz was from the same stock but aged 15 days prior to use. Also, dipalmitoyl lecithin (DPL), the major constituent of lung surfactant coating the alveoli surfaces, was coated onto the particles. Release of O2- was measured after 40 and 180 min of direct contact with quartz dust (225 ug/ml). Fresh quartz was more reactive than aged quartz, as indicated by the 78 and 63% increase after 40 and 180 min, respectively. A time delay and suppression of 02- release was observed in the ability of the DPL-coated fresh or stale dust to stimulate PAM. These results supported the hypothesis that the natural process of surfactant coating of inhaled particles in the alveoli may play a role in their phagocytic removal by PAM. [Description provided by NIOSH]
• Subjects:
  Blood Dust Lung Macrophages Occupational Health Quartz Respiratory System Safety Silicon Dioxide
• Keywords:
  Alveolar Cells In Vitro Study Lung Cells Quartz Dust Respirable Dust
• ISBN:
  9780873350983
• Publisher:
  Society for Mining, Metallurgy, and Exploration, Inc.
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  Colorado ; OSHA Region 3 ; OSHA Region 8 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  313-315
• NIOSHTIC Number:
  nn:20049438
• Citation:
  Respirable dust in the mineral industries, proceedings of the 3rd symposium on respirable dust in the mineral industries, October 17-19, 1990, Pittsburgh, Pennsylvania. Frantz RL, Ramani RV, eds. Littleton, CO: Society for Mining, Metallurgy, and Exploration, Inc., 1991 Jan; :313-315
• Contact Point Address:
  Eugene V. Cilento, Department of Chemical Engineering, West Virginia University, Morgantown, WV 26506-6101
• Editor(s):
  Frantz RL; Ramani RV
• Federal Fiscal Year:
  1991
• Performing Organization:
  West Virginia University
• Peer Reviewed:
  False
• Source Full Name:
  Respirable dust in the mineral industries, proceedings of the 3rd symposium on respirable dust in the mineral industries, October 17-19, 1990, Pittsburgh, Pennsylvania
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8bf9031696c629ae5281e3e18b93723755a9285e074546200f98524a72bb452b15acdbcc8442b82c28cb790e26ef6bdd50fbf73193684c03f0f91e739ee115d8
• Download URL:
  https://stacks.cdc.gov/view/cdc/203883/cdc_203883_DS1.pdf
• File Type:
  [PDF - 2.38 MB ]