Genotoxicity from as-produced and post-production modified multi-walled carbon nanotubes

Details

• Personal Author:
  Bishop L ; Bonner J ; Cena L ; Erdely A ; Kashon M ; Lowry D ; McClure C ; Mitchell C ; Parsons G ; Reynolds S ; Sargent L ; Siegrist K
• Description:
  Pristine, as-produced multi-walled carbon nanotubes (AP-MW) are modified with coatings such as polymers (PC-MW) or aluminum oxide (AL-MW) for varied commercial applications. In order to determine the potential effect of coating on genotoxicity, we measured the genotoxic response of AP-MW from three companies (AP-MW1, 2 and 3) and their coated counterparts (PC-MW1 & 2 and AL-MW3). Genotoxicity was measured in immortalized (BEAS-2B) and primary lung epithelial cells (SAEC) either 24 or 72 hours after exposure using micronuclei assay with pancentromere staining, mitotic spindle analysis, and cell cycle analysis. There was significant necrosis at the highest dose of 24 microg/mL with the exception of AP-MW1. All MWs produced significantly greater amounts of micronuclei after exposure to 2.4 microg/mL in both cell types, however, there was a significant difference between AP-MW2 and PC-MW2 in the BEAS-2B cell. Genetic damage was due to both clastogenic and aneugenic effects in both cell types. Analysis of the mitotic spindle demonstrated a dose-dependent increase of mitotic spindle aberrations that were predominately monopolar. No significant apoptosis was observed in the BEAS-2B cell type. SAEC cells exposed to AL-MW3 showed significant apoptosis. Exposure to 2.4 microg/mL of each material produced significant arrests in the G1 and G2 phases of the cell cycle. These data indicate that all tested materials produced a genotoxic response. The coating did not affect the response in comparison to their respective as-produced counterpart. [Description provided by NIOSH]
• Subjects:
  Aluminum Compounds Aluminum Oxide Biological Assay Environmental Exposure Laboratories Lung Occupational Health Polymers Respiratory System Safety Toxicology

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• Keywords:
  Aluminum Oxide Analytical Processes Bioassays Carbon Nanotubes Cell Cycle Cell Damage Cell Type Cellular Reactions Clastogens Coatings Dose Response Exposure Assessment Exposure Levels Genotoxic Effects Genotoxicity Laboratory Testing Lung Cells Multi-walled Carbon Nanotubes MWCNT Nanotechnology Nanotoxicology Nanotubes

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; North Carolina ; OSHA Region 3 ; OSHA Region 4 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  156
• Issue:
  1
• NIOSHTIC Number:
  nn:20049404
• Citation:
  Toxicologist 2017 Mar; 156(1):125
• CAS Registry Number:
  Alumina (CAS RN 1344-28-1) ; Carbon nanotubes (CAS RN 308068-56-6)
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 56th Annual Meeting and ToxExpo, March 12-16, 2017, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:58cb9ea05a0ffa03a33b6c26c84d37548f25dec23cc076a342e4b7933bf45ba8f79fe3ea5597542a14b37dd043252b1ba3e3a7a0231926d5ab866f4f00fb85b3
• Download URL:
  https://stacks.cdc.gov/view/cdc/203856/cdc_203856_DS1.pdf
• File Type:
  [PDF - 944.10 KB ]