Dose response of multi-walled carbon nanotube-induced lung tumors

Details

• Personal Author:
  Andrew M ; Battelli L ; Castranova, Vincent ; Chen BT ; Endo M ; Hubbs, Ann F. ; Kashon ML ; Lowry D ; McKinnney W ; Porter DW ; Reynolds SH ; Sargent LM ; Siegrist K ; Staska L ; Tsuruoka S
• Description:
  Mitsui-7 MWCNTs are strong lung tumor promoters in B6C3F1 mice. B6C3F1 mouse lung tumors have many molecular and morphological similarities to human pulmonary tumors. In previous work, we have demonstrated that exposure to inhaled Mitsui-7 following exposure to a DNA damaging agent caused potent promotion of lung tumors. To investigate a possible threshold for Mitsui-7-induced carcinogenesis, we exposed B6C3F1 mice to a single dose of either methylcholanthrene (MC, 10 microg/g BW, i.p.) or vehicle (corn oil). One week after i.p. injections, mice were exposed by inhalation to MWCNTs (5 mg/m3, 5 hours/day, 5 days/week) or filtered air (controls) for a total of 2, 5 or 10 days. At 17 months post-exposure, mice were euthanized and examined for lung tumor formation. Thirty six percent of the filtered air controls, 33% of the MWCNT-exposed, and 47% of the MC-exposed, had a mean of 0.33, 0.33 and 0.4 tumors per mouse, respectively. By contrast, 94% of the mice which received MC followed by 10 days MWCNT had an average of 2.9 tumors per mouse while 81% of the mice exposed to 5 days of MWCNTs had 1.9 tumors per mouse, and 73% of the mice exposed to 2 days of MWCNTs had 1.2 tumors per mouse. Additionally, mice exposed to MWCNTs or MC followed by MWCNTs had larger tumor volumes than their corresponding air-exposed control groups. Preliminary data indicate a dose response in the percent of animals with tumors as well as the number of tumors per animal following exposure to MC and MWCNTs. In this study, mouse MWCNT lung burden approximates feasible human occupational exposures. [Description provided by NIOSH]
• Subjects:
  Animals Carcinogens Environmental Exposure Laboratories Lung Lung Diseases Neoplasms Occupational Exposure Occupational Health Respiratory System Respiratory Tract Diseases Safety Toxicology

  More +
• Keywords:
  Carbon Nanotubes Carcinogenesis Dose Response Exposure Assessment Exposure Levels Humans Inhalation Laboratory Animals Laboratory Testing Lung Burden Lung Disorders Multi-walled Carbon Nanotubes MWCNT Nanotechnology Nanotoxicology Nanotubes Occupational Exposure Tumors

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  22
• Volume:
  156
• Issue:
  1
• NIOSHTIC Number:
  nn:20049374
• Citation:
  Toxicologist 2017 Mar; 156(1):22
• CAS Registry Number:
  3-Methylcholanthrene (CAS RN 56-49-5) ; Carbon nanotubes (CAS RN 308068-56-6)
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 56th Annual Meeting and ToxExpo, March 12-16, 2017, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:33f048a44a7e9be32420fb48f4c4a79929413007f8f57376afa1b1ba694e830b7a9706098e7e33d32835e861b2d8b2bdf94877248edc5455ab52c259a5187093
• Download URL:
  https://stacks.cdc.gov/view/cdc/203838/cdc_203838_DS1.pdf
• File Type:
  [PDF - 943.28 KB ]