Latex sensitization by dermal exposure can lead to airway hyperreactivity

Details

• Personal Author:
  Howell MD ; Mead BJ ; Weissman, David N.
• Description:
  Using non-powdered, low-protein natural rubber latex (NRL) gloves has been shown to reduce the elicitation of respiratory symptoms in latex-allergic individuals; however, the role of dermal exposure in the induction of sensitization is not completely understood. Objective: These studies were conducted to (1) determine levels of NRL protein in gloves currently in use and (2) evaluate, using a murine model, the potential for dermal exposure to induce NRL sensitization and subsequent airway hyperreactivity upon respiratory challenge. Methods: Total extractable protein and NRL allergen levels were evaluated from 38 glove samples using the Lowry and CAP inhibition assays, respectively. BALB/c mice were dermally exposed to non-ammoniated latex (NAL, 6.25-25 ug) 5 days/week for 13 weeks and monitored weekly/biweekly for IgE levels. Airway hyperreactivity was determined following respiratory challenge with methacholine (MCH) or NAL proteins on days 60 and 93, respectively. Results: Glove total protein and NRL allergen levels ranged from below the limit of detection to 946 ug/g and from 0.002 to 112 ug/g, respectively. Mice demonstrated dose-dependent increases in total serum IgE levels by day 58 with increased airway hyperreactivity observed upon respiratory challenge with MCH (day 60) or NAL proteins (day 93). Conclusions: These studies investigated the continued use of gloves with high levels of total extractable protein and NRL allergen. The potential for dermal exposure to induce NRL-specific IgE and airway hyperreactivity upon respiratory challenge suggests there should be continued concern regarding the induction of sensitization in individuals using non-powdered latex gloves [Description provided by NIOSH]
• Subjects:
  Hypersensitivity Occupational Health Personal Protective Equipment Protective Clothing Safety
• Keywords:
  Airway Hyperreactivity Allergens Allergic Reactions Allergies Analytical Processes Author Keywords: Latex Allergy Dermal Exposure Gloves IgE Latex Latex Allergies Murine Model Natural Products Natural Rubber Latex Personal Protective Equipment Protective Clothing

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• ISSN:
  1018-2438
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; OD - Office of the Director
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  204-211
• Volume:
  128
• Issue:
  3
• NIOSHTIC Number:
  nn:20049323
• Citation:
  Int Arch Allergy Immunol 2002 Jul; 128(3):204-211
• Contact Point Address:
  Dr. B. Jean Meade, 1095 Willowdale Road, Morgantown, WV 26505 (USA)
• Email:
  bhm8@cdc.gov
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  International Archives of Allergy and Immunology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:de5bd09987ee7e883b6682d8049b42595102a9abb50d9bf22fc73a034558fc5f05907408c63cfd8354e9654f0bf606bbd445f8084fd5faead8d16aa1ea9ba199
• Download URL:
  https://stacks.cdc.gov/view/cdc/203796/cdc_203796_DS1.pdf
• File Type:
  [PDF - 105.42 KB ]