Percutaneous Sensitization to Hev B Proteins in Health Care Workers (HCW) Reporting Allergic Reactions to Natural Rubber Latex (NRL) Gloves: Preliminary Results

Details

• Personal Author:
  Berendis B ; Bernstein C ; Bernstein DI ; Biagini, Raymond E. ; Hamilton R ; Karnani R ; Murphy K ; Yeang HY
• Description:
  Previous evaluation of in vivo sensitization to NRL Hev b proteins has utilized recombinant antigens (Yip et al. Int Arch Allergy Immunol 2000;121:293). The aim of this study is to evaluate skin prick test (spt) reactivity to Hev b proteins 1,2,3,4,6,7b, 7c isolated and purified from non-ammoniated latex (NAL) and to a recombinant Hev b5, in 130 HCWs with cutaneous sensitization to NAL. Preliminary results are presented in 21 HCWs including: 16 NAL spt + HCWs with clinical allergic reactions to NRL gloves (Group 1); and 5 HCWs with clinical reactions to NRL gloves who were spt neg. to NAL (Group 2). Endpoint prick test threshold concentrations (EPC) were determined with 7 increasing concentrations (I x 10(-6) to Img/ml of NRL) and Hev b proteins (5xl0(-5) to 50 ug protein/ml). All subjects were atopic. Fifteen of 16 Group 1 pts. had EPCs for Hev b proteins ranging between 5xl0(-5) and 50ug/ml of NAL. More than 50% of Group I pts. were spt + to Hev b 2 (69%), Hev b 4 (56%), Hev b 5 (69%), Hev b 6 (81 %), Hev b 7b(8l%), Hev b7c (62%) but not to Hev b 1 (25%) and Hev b 3 (31 %). In Group 1, 8/15 pts. were spt + to at least 4/7 Hev b proteins. Interestingly, all Group 2 pts. who were spt neg. to NAL were spt + to at least one Hev b protein with 4/5 reacting to Hev b 7. To date, this study shows the highest frequency of skin test reactivity to Hev b 6 and 7b. Hev b skin test reagents could be sensitive markers of NRL sensitization in symptomatic HCWs exposed to latex. However, extensive validation studies with these antigens are required. [Description provided by NIOSH]
• Subjects:
  Health Personnel Hypersensitivity Occupational Health Personal Protective Equipment Protective Clothing Safety
• Keywords:
  Allergens Allergic-reactions Allergies Gloves Health-care Health-care-personnel Personal-protection Personal-protective-equipment Protective-clothing
• ISSN:
  0091-6749
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Maryland ; Ohio ; OSHA Region 3 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  107
• Issue:
  2
• NIOSHTIC Number:
  nn:20033713
• Citation:
  J Allergy Clin Immunol 2001 Feb; 107(2)(Suppl):S322
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• Supplement:
  S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4c0caed8932206369d3515a3683730cc3cd843812e604ae7a98dd6dc4444cd4aa5c316e4d4f013c418944f36446b92df2cc810309dac5134b95e7cb56af986a0
• Download URL:
  https://stacks.cdc.gov/view/cdc/189179/cdc_189179_DS1.pdf
• File Type:
  [PDF - 237.52 KB ]