Quantitative PCR analysis and protein distribution of drug transporter genes in the rat cochlea

Details

• Personal Author:
  Ding D ; Jamesdaniel S ; Manohar S ; Roth JA ; Salvi R ; Seigel GM
• Description:
  Membrane transporters can be major determinants in the targeting and effectiveness of pharmaceutical agents. A large number of biologically important membrane transporters have been identified and localized to a variety of tissues, organs and cell types. However, little is known about the expression of key membrane transporters in the inner ear, a promising site for targeted therapeutics, as well as a region vulnerable to adverse drug reactions and environmental factors. In this study, we examined the levels of endogenous membrane transporters in rat cochlea by targeted PCR array analysis of 84 transporter genes, followed by validation and localization in tissues by immunohistochemistry. Our studies indicate that several members of the SLC, VDAC and ABC membrane transporter families show high levels of expression, both at the RNA and protein levels in the rat cochlea. Identification and characterization of these membrane transporters in the inner ear have clinical implications for both therapeutic and cytotoxic mechanisms that may aid in the preservation of auditory function. [Description provided by NIOSH]
• Subjects:
  Biopharmaceutics Cytotoxins Energy Metabolism Hearing Immune System Laboratories Occupational Health Safety
• Keywords:
  Analytical Instruments Auditory Protection Author Keywords: Membrane Transporter Biological Transport Cellular Transport Mechanism Cochlea Cytotoxicity Gene Expression Gene Regulation Histochemical Analysis Immunochemistry Immunohistochemistry Inner Ear Laboratory Animals Membrane Filters Metabolism PCR Array Pharmaceuticals Proteins Quantitative Analysis Ribonucleic Acids RNA Therapeutic Agents Tissue Culture

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• ISSN:
  0378-5955
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  46-54
• Volume:
  332
• NIOSHTIC Number:
  nn:20049097
• Citation:
  Hear Res 2016 Feb; 332:46-54
• Contact Point Address:
  Jerome A. Roth, Department of Pharmacology and Toxicology, 11 Cary Hall, University at Buffalo, Buffalo, NY 14214, United States
• Email:
  jaroth@buffalo.edu
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  State University of New York - Buffalo
• Peer Reviewed:
  True
• Start Date:
  20120901
• Source Full Name:
  Hearing Research
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:78d57118655d32fa702da21b22b4cdce57086f76e091b6fb2cccdd49f7a1ab029cb662afc35737ce39788879b94425a63e703ab00de2137c470d863e24d56092
• Download URL:
  https://stacks.cdc.gov/view/cdc/203670/cdc_203670_DS1.pdf
• File Type:
  [PDF - 5.82 MB ]