Protective effect of N-acetyl-L-cysteine (L-NAC) against styrene-induced cochlear injuries

Details

• Personal Author:
  Bielefeld EC ; Chen G-D ; Henderson D ; Hu BH ; Yang WP
• Description:
  CONCLUSION: Styrene exposure causes hair cell death through both apoptotic and necrotic pathways and treatment with N-acetyl-L-cysteine (L-NAC) reduces styrene ototoxicity. OBJECTIVE: Exposure to styrene causes hearing loss and hair cell death in the middle frequency region in the cochlea. The current study was designed to examine the cell death pathways and the protective effect of L-NAC against styrene-induced cochlear injuries. MATERIALS AND METHODS: Seventeen rats were exposed to styrene by gavage at 400 mg/kg 5 days per week for 3 weeks. Nine of the styrene-treated rats received L-NAC by intraperitoneal injection (325 mg/kg), and the remaining eight rats received saline injections as controls. The styrene-induced hearing loss was assessed by auditory brainstem responses (ABRs). Apoptotic, necrotic, and missing hair cells were quantified using combined methods, including nuclear staining with propidium iodide, F-actin staining with FITC-phalloidin, and the TUNEL assay. RESULTS: The styrene exposure caused a threshold shift of 15+/-4.3 dB. Both apoptosis and necrosis were involved in the pathogenesis of the cochlear lesion, but apoptosis appeared to be the major cell death pathway leading to the styrene ototoxicity. Treatment with L-NAC reduced the number of missing and dying outer hair cells (OHCs) and reduced the styrene-induced hearing loss. [Description provided by NIOSH]
• Subjects:
  Animals Energy Metabolism Hearing Loss Hearing Loss, Noise-Induced Laboratories Occupational Health Safety Solvents Styrene
• Keywords:
  Antioxidant Apoptosis Auditory-system Author Keywords: Styrene Cell-damage Cell-function Cochlea Exposure-levels Exposure-limits Hearing-disorders Hearing-impairment Hearing-loss Laboratory-animals Metabolism Necrosis Pharmacology Rat Styrenes

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• ISSN:
  0003-2670
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  129
• Issue:
  10
• NIOSHTIC Number:
  nn:20039195
• Citation:
  Acta Otolaryngol 2009 Oct; 129(10):1036-1043
• Contact Point Address:
  Guang-Di Chen, SUNYat Buffalo, 137 Cary Hall, 3435 Main Street, Buffalo, NY 14214
• Email:
  gchen7@buffalo.edu
• Federal Fiscal Year:
  2010
• Performing Organization:
  State University of New York at Buffalo
• Peer Reviewed:
  True
• Start Date:
  20050501
• Source Full Name:
  Acta Oto-Laryngologica
• End Date:
  20090430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:26a648783c31ce56c53ad39af2f8e0a0838c4aec2abd710fb133b94656c5b14a184846b3ef0fc68ac3585cb879f9733cc73293a80fff57561cbefd52aa6ab216
• Download URL:
  https://stacks.cdc.gov/view/cdc/192633/cdc_192633_DS1.pdf
• File Type:
  [PDF - 385.47 KB ]