Hollow carbon spheres trigger inflammasome-dependent IL-1β secretion in macrophages

Details

• Personal Author:
  Andón FT ; Fadeel B ; Gessner I ; Hultenby K ; Mathur S ; Mukherjee SP ; Shvedova AA ; Wortmann L ; Xiao L
• Description:
  It is disputed whether inflammasome activation leading to secretion of pro-inflammatory interleukin (IL)-1β in macrophages transpires independently of cell death or whether the two processes are linked. Here, we synthesized hollow carbon spheres (HCS) and investigated their effects on primary human monocyte-derived macrophages (HMDM); short (500 nm) non-functionalized single-walled carbon nanotubes (SWCNT) were included for comparison. HCS (250 nm) were readily taken up by HMDM and induced ROS production, but did not trigger a loss of cell viability. However, a dose- and time-dependent release of IL-1β was detected in lipopolysaccharide (LPS)-primed macrophages upon exposure to HCS, while SWCNT-induced secretion of IL-1β was less pronounced. HCS-triggered IL-1β secretion was cathepsin B- and caspase-1-dependent, and was accompanied by a reduction in intracellular K+. Furthermore, cytokine secretion was reduced following treatment with the antioxidant, N-acetylcysteine, and cytochalasin D, an inhibitor of actin polymerization. HCS also triggered IL-1β release in LPS-primed THP.1 cells, but not in THP.1 cells with silencing of ASC, NLRP3, or caspase-1 expression, providing evidence that IL-1β was elicited through NLRP3 inflammasome activation. These studies shed light on the effects of HCS on primary macrophages, and show that spherical carbon-based nanoparticles are potent inflammasome activators. [Description provided by NIOSH]
• Subjects:
  Antioxidants Blood Immune System Macrophages Occupational Health Safety
• Keywords:
  Cell Death Cytokines Immune Reaction Nanoparticles Reactive Oxygen Species ROS Single-walled Carbon Nanotubes SWCNT
• ISSN:
  0008-6223
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  243-251
• Volume:
  113
• NIOSHTIC Number:
  nn:20049014
• Citation:
  Carbon 2017 Mar; 113:243-251
• Contact Point Address:
  Bengt Fadeel, Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm; 17177, Sweden
• Email:
  Bengt.Fadeel@ki.se
• CAS Registry Number:
  Carbon nanotubes (CAS RN 308068-56-6)
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Mining
• Peer Reviewed:
  True
• Source Full Name:
  Carbon
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ddfd9b00c446f0e66d14cfb7286d2f2eaa2d72cbc4612a76729906c5ba26c3fc0b1c25ae13435aca928b394be3339e4d84ab9ea863ca7c7d20e6fd624159fa96
• Download URL:
  https://stacks.cdc.gov/view/cdc/203610/cdc_203610_DS1.pdf
• File Type:
  [PDF - 1.48 MB ]