Single-frequency LCR databridge impedance measurements as surrogate measures for the integrity of human skin

Details

• Personal Author:
  Bunge AL ; Orazem ME ; White EA
• Description:
  Percutaneous absorption data are required for risk assessments of potentially toxic chemicals. In-vitro measurements of human skin can be used to avoid testing on human volunteers or animals. However, the collection and handling of excised skin can introduce damage, which may affect the percutaneous absorption measurements. Therefore, testing whether the barrier function of skin samples has sufficient integrity for meaningful measurements of in-vitro chemical permeability is common and usually required when data are generated for regulatory purposes. Measurement of skin impedance is faster and less expensive than measuring tritiated water permeation. For the same reason, in-vitro impedance measurements are also used to identify chemicals and chemical mixtures that cause irreversible (or corrosive) damage to the skin. Impedance measurements of in-vitro skin samples are commonly determined using an LCR Databridge. According to their manufacturers, these instruments measure inductance (L), capacitance (C) or resistance (R) in either a parallel (PAR) or series (SER) mode determined at one of two user-selected frequencies, usually 100 or 1000 Hz. Nearly always, the reported resistance values measured by PAR and SER modes (i.e., RPAR and RSER, respectively) at a given frequency are different. Also, RPAR and RSER, determined at different frequencies, are different, clearly indicating that these instruments do not report the DC skin resistance. In this work, a large quantity of skin impedance data was used to demonstrate the relationships among the LCR Databridge results and skin resistivity or integrity. [Description provided by NIOSH]
• Subjects:
  Chemistry Occupational Health Safety Skin Skin Absorption
• Keywords:
  Chemical Properties Corrosive Materials Humans In Vitro Studies In Vitro Study Risk Assessment Skin Absorption Skin Exposure Toxic Effects
• Publisher:
  The Electrochemical Society
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Colorado ; Florida ; OSHA Region 4 ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20048696
• Citation:
  ECS Meeting Abstracts, 220th ECS Meeting, Oct 9-14, 2011, Boston, Massachusetts. 2011 Oct; MA2011-02:2606
• Contact Point Address:
  Mark E. Orazem, 2Department of Chemical Engineering, University of Florida, Florida 32611
• Email:
  meo@che.ufl.edu
• Federal Fiscal Year:
  2012
• Performing Organization:
  Colorado School of Mines
• Peer Reviewed:
  False
• Start Date:
  20040901
• Source Full Name:
  ECS Meeting Abstracts, 220th ECS Meeting, Oct 9-14, 2011, Boston, Massachusetts
• End Date:
  20090331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:333dc0d13a3de7125c0d6dba6a922a097766fb09f7b2d3b2503943988c408a955844c677be9b9a273a3eee71d915f1b4054ce7e53f9873896bc4cb4c1adf7a15
• Download URL:
  https://stacks.cdc.gov/view/cdc/203389/cdc_203389_DS1.pdf
• File Type:
  [PDF - 101.11 KB ]