Nanosilver induces minimal lung toxicity or inflammation in a subacute murine inhalation model

Details

• Personal Author:
  Adamcakova-Dodd A ; Grassian VH ; Kim JS ; O'Shaughnessy PT ; Park H ; Stebounova LV ; Thorne PS
• Description:
  Background: There is increasing interest in the environmental and health consequences of silver nanoparticles as the use of this material becomes widespread. Although human exposure to nanosilver is increasing, only a few studies address possible toxic effect of inhaled nanosilver. The objective of this study was to determine whether very small commercially available nanosilver induces pulmonary toxicity in mice following inhalation exposure. Results: In this study, mice were exposed sub-acutely by inhalation to well-characterized nanosilver (3.3 mg/m3, 4 hours/day, 10 days, 5 +/- 2 nm primary size). Toxicity was assessed by enumeration of total and differential cells, determination of total protein, lactate dehydrogenase activity and inflammatory cytokines in bronchoalveolar lavage fluid. Lungs were evaluated for histopathologic changes and the presence of silver. In contrast to published in vitro studies, minimal inflammatory response or toxicity was found following exposure to nanosilver in our in vivo study. The median retained dose of nanosilver in the lungs measured by inductively coupled plasma - optical emission spectroscopy (ICP-OES) was 31 µg/g lung (dry weight) immediately after the final exposure, 10 µg/g following exposure and a 3-wk rest period and zero in sham-exposed controls. Dissolution studies showed that nanosilver did not dissolve in solutions mimicking the intracellular or extracellular milieu. Conclusions: Mice exposed to nanosilver showed minimal pulmonary inflammation or cytotoxicity following sub-acute exposures. However, longer term exposures with higher lung burdens of nanosilver are needed to ensure that there are no chronic effects and to evaluate possible translocation to other organs. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Exposure Hazardous Substances Laboratories Lung Occupational Health Respiratory System Risk Assessment Risk Factors Safety Silver Toxicology Workplace

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• Keywords:
  Cell Function Cellular Effects Cellular Function Cytokines Exposure Levels Health Effects Humans Inhalation Inhalation Studies Laboratory Animals Lung Tissue Nanomaterials Nanoparticles Nanotoxicology Proteins Risk Factors Silver Compounds Toxic Effects Toxins Work Environment Workers

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• ISSN:
  1743-8977
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Iowa ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  5
• Volume:
  8
• NIOSHTIC Number:
  nn:20048408
• Citation:
  Part Fibre Toxicol 2011 Jan; 8:5
• Contact Point Address:
  Peter S Thorne, Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52242, USA
• Email:
  peter-thorne@uiowa.edu
• Federal Fiscal Year:
  2011
• Performing Organization:
  University of Iowa
• Peer Reviewed:
  True
• Start Date:
  20080401
• Source Full Name:
  Particle and Fibre Toxicology
• End Date:
  20120331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6f43e359fe3e04d67c46b2bb31ab764b6a4affa7707811a37391ea133785a8e63c45cbe9e5f9fc672913ddf7d87dbcf5ef75f91e3deea87d842fdd20c276033b
• Download URL:
  https://stacks.cdc.gov/view/cdc/203167/cdc_203167_DS1.pdf
• File Type:
  [PDF - 2.50 MB ]